Department of Chemistry, Sree Narayna College, Chempazhanthy, University of Kerala, Thiruvananthapuram-695587, India.
Institut für Anorganische Chemie und Strukturchemie, Heinrich-Heine-Universität Düsseldorf, Universitätsstraße 1, D-40225 Düsseldorf, Germany.
Dalton Trans. 2022 May 24;51(20):7748-7762. doi: 10.1039/d2dt00128d.
Lysosomes are essential acidic cytoplasmic membrane-bound organelles in human cells that play a critical role in many cellular events. A comprehensive understanding of lysosome-specific imaging can ultimately help us to design robust organelle-targeting therapeutic reagents for various underlying human diseases. Luminescent lanthanide molecular materials serve as an important and upcoming class of probes for cellular imaging applications with unique luminescent photophysical features such as sharp emission profiles from the visible to near-infrared spectral regions, long decay lifetimes, attractive quantum yields, large Stokes shifts, and a low propensity to photobleaching. For the last few years, a wide variety of lysosome-targeting luminescent lanthanide probes have been engineered and utilized for the imaging of hypochlorous acid and nitric oxide at the sub-cellular level and these advances are summarized in this review. The design strategies of lanthanide molecular probes, co-localization detection and lysosomal probity assay methods are briefly highlighted. Finally, the future challenges in the development of lysosome-targeting luminescent lanthanide complexes are outlined and emphasized to inspire the design of a new generation of organelle-targeting metal complexes.
溶酶体是人类细胞中重要的酸性细胞质膜结合细胞器，在许多细胞事件中发挥着关键作用。全面了解溶酶体特异性成像最终将有助于我们设计针对各种潜在人类疾病的稳健细胞器靶向治疗试剂。发光镧系分子材料是一类重要的新兴探针，可用于细胞成像应用，具有独特的发光光物理特性，如从可见到近红外光谱区域的尖锐发射谱、长衰减寿命、有吸引力的量子产率、大斯托克斯位移和低光漂白倾向。在过去的几年中，已经设计并利用了多种溶酶体靶向发光镧系探针来对亚细胞水平的次氯酸和一氧化氮进行成像，本综述总结了这些进展。简要强调了镧系分子探针的设计策略、共定位检测和溶酶体探针分析方法。最后，概述并强调了开发溶酶体靶向发光镧系配合物的未来挑战，以激发新一代细胞器靶向金属配合物的设计。
