State Key Laboratory of Organic-Inorganic Composites, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, People's Republic of China.
Foshan (Southern China) Institute for New Materials, Foshan 528200, Guangdong, People's Republic of China.
Biomed Mater. 2022 May 10;17(4). doi: 10.1088/1748-605X/ac6b72.
This study intends to improve the antibacterial and mineralization performance of photocurable dental resin composites (DRCs) to reduce the possibility of repair failure caused by secondary caries. To the end, functionalized hydroxyapatite (HAp), including Zn-doped (Zn/HAp) and Sr-doped HAp (Sr/HAp), were added into the bisphenol A glycidyl methacrylate and triethylene glycol dimethacrylate mixture, providing the DRCs with antibacterial and mineralization capacity, respectively. By controlling the total amount of inorganic filler at 70 wt%, these HAp powders were introduced into the resin matrix with barium glass powder (BaGP), while the ratios of HAp to aGP varied from 0:70 to 8:62. And the 8 wt% of HAp could be pure HAp, Zn/HAp, Sr/HAp, or Zn/HAp +Sr/HAp in different ratios (i.e. 2:6, 4:4, 6:2). Though the fillers varied, the obtained DRCs displayed similar micro-morphology, flexural strength (∼110 MPa) and modulus (∼7 GPa), and Vickers hardness (∼65). When the doping amounts of Sr/Znreached 15 mol% of Cain the Sr/HAp and Zn/HAp, the DRCs displayed a high antibacterial activity by killing ∼95%, and induced rich mineral deposition on surface in simulated body fluid. The incorporation of the Zn/HAp and Sr/HAp into the DRCs did not cause significant cytotoxicity, with L929 fibroblasts remaining >99% viability as cultured in extracts made from the DRCs. Therein, the DRC preparations containing both Zn/HAp and Sr/HAp have achieved improvements in both the biomineralization and antibacterial performance, as well as, having sufficient mechanical properties and excellent biocompatibility for dental restoration.
本研究旨在提高光固化牙科树脂复合材料（DRC）的抗菌和矿化性能，以降低继发龋引起的修复失败的可能性。为此，在双酚 A 缩水甘油甲基丙烯酸酯和三乙二醇二甲基丙烯酸酯混合物中添加了功能化羟基磷灰石（HAp），包括掺锌（Zn/HAp）和掺锶（Sr/HAp）的 HAp，分别为 DRC 提供抗菌和矿化能力。通过控制无机填料的总量为 70wt%，将这些 HAp 粉末与钡玻璃粉（BaGP）一起引入树脂基质中，而 HAp 与 aGP 的比例从 0:70 变化到 8:62。并且 8wt%的 HAp 可以是纯 HAp、Zn/HAp、Sr/HAp 或 Zn/HAp+Sr/HAp 以不同的比例（即 2:6、4:4、6:2）存在。尽管填料不同，但所得到的 DRC 显示出相似的微观形貌、弯曲强度（约 110MPa）和模量（约 7GPa）以及维氏硬度（约 65）。当 Sr/Zn 的掺杂量达到 Sr/HAp 和 Zn/HAp 中 Ca 的 15mol%时，DRC 表现出高抗菌活性，杀死了约 95%的细菌，并在模拟体液中诱导了丰富的表面矿化沉积。将 Zn/HAp 和 Sr/HAp 掺入 DRC 中不会导致显著的细胞毒性，当在从 DRC 制备的提取物中培养时，L929 成纤维细胞的存活率仍保持在>99%。在此，含有 Zn/HAp 和 Sr/HAp 的 DRC 制剂在生物矿化和抗菌性能方面均得到了提高，同时具有足够的机械性能和良好的生物相容性，可用于牙科修复。
