Institute of New Carbon Materials, Taiyuan University of Technology, 79 West Yingze Road, Taiyuan 030024, China.
State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, 1295 Dingxi Road, Shanghai 200050, China.
Dent Mater. 2020 Jun;36(6):794-807. doi: 10.1016/j.dental.2020.03.026. Epub 2020 Apr 27.
The purpose of this work was to explore the enhancement effect of zinc doped mesoporous silica nanoparticles (Zn-MSNs), which could form micromechanical interlocking with resin matrix and sustainably release Zn, on the mechanical and antibacterial properties of the dental resin composites.
Zn-MSNs were prepared by a sol-gel method, and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM) and N adsorption/desorption. The mechanical properties of the dental composites reinforced by Zn-MSNs were measured by a universal mechanical testing machine. Antibacterial activities of dental composites were evaluated by both qualitative and quantitative analysis using Streptococcus mutans (S. mutans). The cytotoxicity of the Zn-MSNs filled dental composites was investigated by osteoblasts (OBs).
The synthesized Zn-MSNs possessed good monodispersity with an average particle size of about 138nm. The mechanical properties of the composites gradually increased with the increase of the content of Zn-MSNs. The flexural strength, flexural modulus, compressive strength and micro-hardness of the composites containing 15wt% Zn-MSNs were 31.21%, 50.47%, 53.83% and 26.79% higher than the samples with no Zn-MSNs, respectively. The antibacterial performance was significantly improved by the addition of Zn-MSNs and the antibacterial rate of the composite with 15wt% of Zn-MSNs reached 100%. Cytotoxicity tests revealed that all the composites were biocompatible during OBs incubation.
The prepared Zn-MSNs can effectively improve the mechanical and antibacterial properties of the dental resin composites.
本研究旨在探讨锌掺杂介孔硅纳米粒子（Zn-MSNs）的增强效果，Zn-MSNs 可与树脂基质形成机械联锁，并可持续释放 Zn，从而提高牙科树脂复合材料的力学性能和抗菌性能。
采用溶胶-凝胶法制备 Zn-MSNs，通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）和 N2 吸附/脱附对其进行表征。采用万能材料试验机测量 Zn-MSNs 增强牙科复合材料的力学性能。采用定性和定量分析方法（使用变形链球菌（S. mutans））评估牙科复合材料的抗菌活性。通过成骨细胞（OBs）研究填充 Zn-MSNs 的牙科复合材料的细胞毒性。
合成的 Zn-MSNs 具有良好的单分散性，平均粒径约为 138nm。复合材料的力学性能随 Zn-MSNs 含量的增加而逐渐提高。含有 15wt%Zn-MSNs 的复合材料的弯曲强度、弯曲模量、压缩强度和显微硬度分别比不含 Zn-MSNs 的样品提高了 31.21%、50.47%、53.83%和 26.79%。添加 Zn-MSNs 可显著提高抗菌性能，复合材料中添加 15wt%Zn-MSNs 的抗菌率达到 100%。细胞毒性试验表明，在 OBs 孵育期间，所有复合材料均具有生物相容性。
制备的 Zn-MSNs 可有效提高牙科树脂复合材料的力学性能和抗菌性能。
