利用ATAC-Seq技术探索小鼠上胚层干细胞中的染色质可及性
Exploring Chromatin Accessibility in Mouse Epiblast Stem Cells with ATAC-Seq.
作者信息
Salehin Nazmus, Santucci Nicole, Osteil Pierre, Tam Patrick P L
机构信息
Embryology Research Unit, Children's Medical Research Institute, University of Sydney, Westmead, NSW, Australia.
Faculty of Medicine and Health, School of Medical Sciences, University of Sydney, Camperdown, NSW, Australia.
出版信息
Methods Mol Biol. 2022;2490:93-100. doi: 10.1007/978-1-0716-2281-0_9.
The assay for transposase-accessible chromatin using sequencing (ATAC-seq) is used to identify open chromatin regions in cells. This can be used to identify putative regulatory regions, determine dynamics and mechanisms of transcription factors when coupled with ChIP-seq and predict interactions between proteins and chromatin. Compared to previous methods, MNase-seq and DNase-seq, ATAC-seq requires only 50,000 cells, orders of magnitude fewer cells. In addition, the ATAC-seq protocol takes one day to progress from cells to sequencing ready libraries.
利用测序技术进行转座酶可及染色质分析（ATAC-seq）用于识别细胞中的开放染色质区域。当与染色质免疫沉淀测序（ChIP-seq）结合使用时，它可用于识别潜在的调控区域、确定转录因子的动态变化和作用机制，并预测蛋白质与染色质之间的相互作用。与之前的方法，如微球菌核酸酶测序（MNase-seq）和脱氧核糖核酸酶测序（DNase-seq）相比，ATAC-seq仅需要50000个细胞，所需细胞数量减少了几个数量级。此外，ATAC-seq实验方案从细胞到准备好用于测序的文库只需一天时间。
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