Kurose Rie, Satoh Takashi, Kurose Akira, Satoh Yo-Ichi, Ishibashi Yasuyuki, Wakai Yuji, Sasaki Tomoyuki, Ishida Kinji, Ogasawara Katsutoshi, Sawai Takashi
Hirosaki University Graduate School of Medicine, Hirosaki, Japan.
Iwate Medical University School of Medicine, Morioka, Japan.
ACR Open Rheumatol. 2022 Jul;4(7):603-612. doi: 10.1002/acr2.11440. Epub 2022 Apr 29.
CD14 dendritic-shaped cells show a dendritic morphology under the electron microscopy and engage in a pseudoemperipolesis phenomenon with lymphocytes. CD90 has been used as a marker of a major subset of fibroblast-like synoviocytes in rheumatoid arthritis (RA). In this study, we investigated the significance of CD90 expression in CD14 dendritic-shaped cells and its correlation with RA chronic inflammation.
Double immunofluorescence staining for CD14 and CD90 was performed in the collected tissues, including 12 active RA synovial tissues. The localization of CD14 CD90 cells, the percentages of CD14 CD90 cells and vascular areas, the degree of synovitis, and clinical data were investigated. Furthermore, CD14 CD90 cells analyzed by flow cytometry (CD14 CD90 cells) were sorted from RA synovial cells, and we examined their potential to differentiate into dendritic cells.
Double immunofluorescence staining showed that CD14 CD90 cells were abundant in RA synovial tissues. The percentages of CD14 CD90 cells and vascular areas correlated with some of the Krenn synovitis scores, but neither showed a strong correlation with RA disease activity parameters. Flow cytometry analysis indicated that CD14 CD90 cells were more abundant in both peripheral blood samples and synovial tissues in patients with active RA. CD14 CD90 cells were more likely to differentiate into dendritic cells in vitro.
CD14 dendritic-shaped cells expressed CD90 in the perivascular areas of RA synovial tissues. These findings suggest that CD14 CD90 dendritic-shaped cells migrate from the peripheral blood to the synovial tissue, the site of inflammation, and may contribute to the chronic inflammation of RA as dendritic progenitor cells.
CD14树突状细胞在电子显微镜下呈现树突形态，并与淋巴细胞发生假包绕现象。CD90已被用作类风湿关节炎（RA）中主要成纤维样滑膜细胞亚群的标志物。在本研究中，我们调查了CD90在CD14树突状细胞中的表达意义及其与RA慢性炎症的相关性。
对收集的组织进行CD14和CD90的双重免疫荧光染色，包括12例活动性RA滑膜组织。研究了CD14 CD90细胞的定位、CD14 CD90细胞和血管区域的百分比、滑膜炎程度及临床数据。此外，从RA滑膜细胞中筛选出通过流式细胞术分析的CD14 CD90细胞（CD14 CD90细胞），并检测它们分化为树突状细胞的潜力。
双重免疫荧光染色显示，RA滑膜组织中CD14 CD90细胞丰富。CD14 CD90细胞和血管区域的百分比与一些Krenn滑膜炎评分相关，但均与RA疾病活动参数无强相关性。流式细胞术分析表明，活动性RA患者外周血样本和滑膜组织中CD14 CD90细胞均更为丰富。CD14 CD90细胞在体外更易分化为树突状细胞。
CD14树突状细胞在RA滑膜组织的血管周围区域表达CD90。这些发现表明，CD14 CD90树突状细胞从外周血迁移至炎症部位滑膜组织，并可能作为树突状祖细胞促进RA的慢性炎症。
