Weill Cornell Medicine, New York, NY, USA.
Department of Pathology and Laboratory Medicine, Weill Cornell Medicine, New York, NY, USA.
Cytokine. 2022 Jun;154:155894. doi: 10.1016/j.cyto.2022.155894. Epub 2022 Apr 25.
To study how severity and progression of coronavirus disease (COVID-19) affect cytokine profiles in pregnant women.
69 third-trimester, pregnant women were tested for COVID-19 infection and SARS-CoV-2 specific IgM and IgG antibodies. Patients were stratified according to SARS-CoV-2 Reverse Transcriptase-PCR (RT-PCR) status and serology (IgM and IgG) status. Cytokines G-CSF, HGF, IL-18, IL-1Ra, IL-2Ra, IL-8, and IP-10 were measured via ELISA. Retrospective chart review for COVID-19 symptoms and patient vitals was conducted, and cytokine levels were compared between SARS-CoV-2 positive and negative cohorts, by seronegative and seropositive infection, by time course since onset of infection, and according to NIH defined clinical severity.
IL-18, IL-1Ra, and IP-10 increased in the 44 RT-PCR positive pregnant women compared to the 25 RT-PCR negative pregnant controls. Elevated cytokine levels were found in early infections, defined by positive RT-PCR and seronegative status, and higher cytokine levels were also associated with more severe disease. By IgM seroconversion, IL-8 and IP-10 returned to levels seen in uninfected patients, while IL-18 levels remained significantly elevated.
Cytokine profiles of third-trimester pregnant women vary with the time course of infection and are correlated with clinical severity.
研究新型冠状病毒疾病（COVID-19）的严重程度和进展如何影响孕妇的细胞因子谱。
对 69 名处于妊娠晚期的孕妇进行了 COVID-19 感染和 SARS-CoV-2 特异性 IgM 和 IgG 抗体检测。根据 SARS-CoV-2 逆转录酶-PCR（RT-PCR）状态和血清学（IgM 和 IgG）状态对患者进行分层。通过 ELISA 测量细胞因子 G-CSF、HGF、IL-18、IL-1Ra、IL-2Ra、IL-8 和 IP-10。对 COVID-19 症状和患者生命体征进行了回顾性图表审查，并比较了 SARS-CoV-2 阳性和阴性队列、血清学阴性和阳性感染、感染后时间以及根据 NIH 定义的临床严重程度之间的细胞因子水平。
与 25 名 RT-PCR 阴性妊娠对照组相比，44 名 RT-PCR 阳性孕妇的 IL-18、IL-1Ra 和 IP-10 增加。在早期感染中发现了升高的细胞因子水平，定义为 RT-PCR 阳性和血清学阴性状态，并且更高的细胞因子水平也与更严重的疾病相关。通过 IgM 血清转化，IL-8 和 IP-10 恢复到未感染患者的水平，而 IL-18 水平仍显著升高。
妊娠晚期孕妇的细胞因子谱随感染时间的变化而变化，并与临床严重程度相关。
