Erickson P A, Fisher S K, Guérin C J, Anderson D H, Kaska D D
Exp Eye Res. 1987 Jan;44(1):37-48. doi: 10.1016/s0014-4835(87)80023-4.
Retinal detachment, separation of the neural retina from the retinal pigment epithelium (RPE), initiates a series of changes in the eye which result in loss of vision if the retina is not rapidly reattached to the RPE. Many of the complex effects of this separation on the cell biology of the retina have yet to be determined. We report here a change in the amount and location of a specific cytoskeletal protein, glial fibrillary acidic protein (GFAP), within Müller cells after retinal detachment. Cat neural retina and RPE were separated by injecting fluid into the extracellular space between the retina and RPE. Normal retinas and retinas detached for 30 days were fixed and embedded for conventional electron microscopy or immunocytochemistry, or homogenized and processed by SDS-PAGE for immunoblot analysis with anti-GFAP. In normal retinas and in attached retinal regions of eyes with retinal detachment, GFAP was detected only in the end feet of the Müller cells as 10 nm diameter filaments and as a diffuse component over the cytoplasm. By contrast, in regions where the retina was detached from the RPE, GFAP was localized throughout the Müller cells in both of these forms. Immunoblots revealed a significant increase in anti-GFAP labeling of a 51,000 MW band from the detached retina.
视网膜脱离，即神经视网膜与视网膜色素上皮（RPE）分离，会引发眼睛内一系列变化，如果视网膜不能迅速重新附着于RPE，将会导致视力丧失。这种分离对视网膜细胞生物学产生的许多复杂影响尚未明确。我们在此报告视网膜脱离后，缪勒细胞内一种特定的细胞骨架蛋白——胶质纤维酸性蛋白（GFAP）的数量和位置发生了变化。通过向猫的视网膜与RPE之间的细胞外间隙注入液体，将神经视网膜与RPE分离。将正常视网膜以及脱离30天的视网膜固定、包埋，用于常规电子显微镜检查或免疫细胞化学分析，或者匀浆后通过SDS-PAGE进行处理，以便用抗GFAP抗体进行免疫印迹分析。在正常视网膜以及视网膜脱离眼中的附着视网膜区域，仅在缪勒细胞的终足中检测到GFAP，呈直径10 nm的细丝状以及细胞质中的弥漫成分。相比之下，在视网膜与RPE分离的区域，GFAP以这两种形式遍布于整个缪勒细胞。免疫印迹显示，脱离视网膜的51,000 MW条带的抗GFAP标记显著增加。
