Department of Medicine, Oregon Health and Science University, Portland, OR, United States.
Pulmonary and Critical Care Medicine, Portland VA Medical Center, Portland, OR, United States.
Front Immunol. 2022 Apr 12;13:869057. doi: 10.3389/fimmu.2022.869057. eCollection 2022.
For most vaccination studies, the assessment of vaccine-induced CD4 and CD8 T cells has relied upon the measurement of antigen-specific polyfunctional cells, typically using recombinant antigen or peptide pools. However, this approach leaves open the question as to whether or not these cells are responsive to the Mtb-infected cell within the context of Mtb infection and hence leaves open the possibility that a key parameter of vaccine immunogenicity may be overlooked. In this review, we discuss the case that these measurements almost certainly over-estimate the capacity of both CD4 and CD8 T cells to recognize the Mtb-infected cell.
对于大多数疫苗研究，评估疫苗诱导的 CD4 和 CD8 T 细胞依赖于抗原特异性多功能细胞的测量，通常使用重组抗原或肽库。然而，这种方法留下了一个问题，即这些细胞是否对感染结核分枝杆菌的细胞有反应，因此有可能忽略了疫苗免疫原性的一个关键参数。在这篇综述中，我们讨论了这些测量方法几乎肯定高估了 CD4 和 CD8 T 细胞识别结核分枝杆菌感染细胞的能力。
