Reinecke M, Schlüter P, Yanaihara N, Forssmann W G
Peptides. 1981;2 Suppl 2:149-56. doi: 10.1016/0196-9781(81)90025-5.
The gastrointestinal tracts of various species chosen as representatives of the seven vertebrate classes were investigated for the occurrence of VIP immunoreactivity. The study was carried out with identically fixed and embedded tissue using the peroxidase-antiperoxidase (PAP) technique after primary incubation with a C-terminally directed antiserum (R 501). In the mammalian and avian species the antiserum failed to demonstrate VIP immunoreactive endocrine cells, while in the gut of all representatives of the lower vertebrate classes such cells were seen. The VIP immunoreactive cells were scattered among the epithelial cells of the entire intestine with a slightly higher density in the duodenum of bony fish, amphibians and reptiles. In contrast, VIP immunoreactivity in enteric nerve fibers and perikarya was found in all species studied except for the cyclostomes where the presence of VIP immunoreactive enteric fibers is questionable. The results may support, at least for mammals and birds, the hypothesis that true VIP is of nervous origin, while the endocrine cells contain VIP-related peptides.
对被选为七个脊椎动物纲代表的不同物种的胃肠道进行了研究，以确定是否存在血管活性肠肽（VIP）免疫反应性。该研究使用相同固定和包埋的组织，在与C末端定向抗血清（R 501）进行初次孵育后，采用过氧化物酶抗过氧化物酶（PAP）技术进行。在哺乳动物和鸟类物种中，该抗血清未能显示出VIP免疫反应性内分泌细胞，而在所有低等脊椎动物纲代表的肠道中都观察到了此类细胞。VIP免疫反应性细胞散布在整个肠道的上皮细胞之间，在硬骨鱼、两栖动物和爬行动物的十二指肠中密度略高。相比之下，除了圆口纲动物外，在所有研究的物种中都发现了肠神经纤维和神经周体中的VIP免疫反应性，圆口纲动物中VIP免疫反应性肠纤维的存在情况尚存在疑问。这些结果至少对于哺乳动物和鸟类而言，可能支持这样一种假说，即真正的VIP起源于神经，而内分泌细胞含有与VIP相关的肽。
