Naskar Atanu, Lee Sohee, Kim Kwang-Sun
Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University Busan 46241 South Korea
RSC Adv. 2020 Jan 8;10(3):1232-1242. doi: 10.1039/c9ra09512h. eCollection 2020 Jan 7.
Infections by multidrug-resistant (MDR) bacteria are one of the most threatening concerns for public health. For this purpose, nanomaterials have emerged with great potential for antibacterial activity. In this paper, we report the synthesis of new Ni-doped zinc oxide (Ni-ZnO or NZO) nanostructures as targeted antibacterial agents for Gram-negative bacteria. A one-pot low-temperature solution process was used with varying compositions containing 2 or 5% Ni relative to Zn, resulting in 2NZO or 5NZO, respectively. X-ray diffractometry, transmission electron microscopy, and X-ray photoelectron spectroscopy were used for material characterization. Further, the antibacterial activity against both Gram-negative [ () and () strains including standard, MDR, and clinical isolates associated with gene] and Gram-positive ( and ) bacteria were evaluated through analysis of zone of inhibition, minimum inhibitory concentration (MIC), and scanning electron microscopy images. Among the prepared nanostructures, the 5NZO sample showed excellent antibacterial activity against MDR strains of and . In addition, samples of NZO generated approximately 7 to 16 times more reactive oxygen species (ROS) in compared to ZnO. Our synthesized nanomaterials have the potential to fight MDR and colistin-resistant Gram-negative bacteria.
耐多药（MDR）细菌感染是公共卫生面临的最具威胁性的问题之一。为此，纳米材料已展现出巨大的抗菌活性潜力。在本文中，我们报告了新型镍掺杂氧化锌（Ni-ZnO或NZO）纳米结构的合成，其作为针对革兰氏阴性菌的靶向抗菌剂。采用一锅低温溶液法，使用相对于锌含有2%或5%镍的不同组成，分别得到2NZO或5NZO。利用X射线衍射、透射电子显微镜和X射线光电子能谱进行材料表征。此外，通过抑菌圈分析、最低抑菌浓度（MIC）和扫描电子显微镜图像，评估了对革兰氏阴性菌[（）和（）菌株，包括标准菌株、耐多药菌株以及与基因相关的临床分离株]和革兰氏阳性菌（和）的抗菌活性。在所制备的纳米结构中，5NZO样品对和的耐多药菌株表现出优异的抗菌活性。此外，与ZnO相比，NZO样品在中产生的活性氧（ROS）大约多7至16倍。我们合成的纳米材料有对抗耐多药和耐黏菌素革兰氏阴性菌的潜力。
