Department of Life and Nanopharmaceutical Sciences and Department of Biology, Kyung Hee University, Seoul 130-701, Republic of Korea.
Biological Resources Utilization Department, National Institute of Biological Resources, Incheon, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Apr;70(4):2305-2311. doi: 10.1099/ijsem.0.004034. Epub 2020 Feb 7.
A novel Gram-negative, obligately aerobic, rod-shaped and non-motile bacterium, designated strain K13M18, was isolated from the intestinal tract of a Korean indigenous fish, oily bitterling (). Strain K13M18 formed creamy-pink colonies on a marine agar plate. Results of phylogenetic analysis based on the 16S rRNA gene sequence similarity indicated that strain K13M18 was most closely related to DRYC-M-16, sharing 97.62 % similarity with that strain. Strain K13M18 belonged to the genus , which formed a cluster with RCRI19, SY72, DRYC-M-16 and DJC in a phylogenetic tree based on the 16S rRNA gene sequences. Strain K13M18 grown optimally in 0 % (w/v) NaCl, at pH 7 and 30 °C, in a marine broth medium. The predominant cellular fatty acids were C 7 and C 6. The major respiratory isoprenoid quinone was ubiquinone Q-10. Polar lipids of strain K13M18 contained phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol, six unidentified aminophospholipids, one unidentified aminolipid and an unidentified lipid. Based on genome sequencing, the DNA G+C content of strain K13M18 was 64.08 mol %, with an average nucleotide identity value, calculated by a comparative genomic analysis of strains K13M18 and DRYC-M-16, of 74.82 %. Based on the phylogenetic, genotypic, and phenotypic information, strain K13M18 is proposed to be a novel species of the genus . The type strain is K13M18 (=KCTC 62659=JCM 33230).
一株新型革兰氏阴性、需氧、杆状、非运动性细菌，被命名为 K13M18 菌株，从韩国本土鱼类油性苦鱼的肠道中分离得到。K13M18 菌株在海洋琼脂平板上形成奶油粉红色的菌落。基于 16S rRNA 基因序列相似性的系统发育分析结果表明，K13M18 菌株与 DRYC-M-16 菌株最为密切相关，相似性为 97.62%。K13M18 菌株属于 属，在基于 16S rRNA 基因序列的系统发育树中，与 RCRI19、SY72、DRYC-M-16 和 DJC 一起形成一个聚类。K13M18 菌株在 0%（w/v）NaCl、pH7 和 30°C 的海洋肉汤培养基中生长最佳。主要的细胞脂肪酸为 C7 和 C6。主要的呼吸异戊二烯醌为泛醌 Q-10。K13M18 菌株的极性脂含有磷脂酰胆碱、磷脂酰甘油、磷脂酰肌醇、双磷脂酰甘油、六种未鉴定的氨基磷脂、一种未鉴定的氨基脂和一种未鉴定的脂。基于基因组测序，K13M18 菌株的 DNA G+C 含量为 64.08mol%，通过比较 K13M18 菌株和 DRYC-M-16 菌株的基因组分析，平均核苷酸同一性值为 74.82%。基于系统发育、基因型和表型信息，K13M18 菌株被提议为属的一个新型种。模式菌株为 K13M18（=KCTC 62659=JCM 33230）。
