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通过内皮细胞分泌的细胞外囊泡，特级初榨橄榄油在平滑肌细胞中比葵花籽油更能促进形成较少动脉粥样硬化的状态。

EVOO Promotes a Less Atherogenic Profile Than Sunflower Oil in Smooth Muscle Cells Through the Extracellular Vesicles Secreted by Endothelial Cells.

作者信息

Santiago-Fernandez Concepción, Rodríguez-Díaz Cristina, Ho-Plagaro Ailec, Gutierrez-Repiso Carolina, Oliva-Olivera Wilfredo, Martin-Reyes Flores, Mela Virginia, Bautista Rocío, Tome Mónicas, Gómez-Maldonado Josefa, Tinahones Francisco J, Garcia-Fuentes Eduardo, Garrido-Sánchez Lourdes

机构信息

Instituto de Investigación Biomédica de Málaga-IBIMA, Málaga, Spain.

Unidad de Gestión Clínica de Aparato Digestivo, Hospital Universitario Virgen de la Victoria, Málaga, Spain.

出版信息

Front Nutr. 2022 Apr 12;9:867745. doi: 10.3389/fnut.2022.867745. eCollection 2022.

DOI:10.3389/fnut.2022.867745
PMID:35495944
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9039400/
Abstract

BACKGROUND

Little is known about the effect of extra virgin olive (EVOO) and sunflower oil (SO) on the composition of extracellular vesicles (EVs) secreted by endothelial cells and the effects of these EVs on smooth muscle cells (SMCs). These cells play an important role in the development of atherosclerosis.

METHODS

We evaluated the effects of endothelial cells-derived EVs incubated with triglyceride-rich lipoproteins obtained after a high-fat meal with EVOO (EVOO-EVs) and SO (SO-EVs), on the transcriptomic profile of SMCs.

RESULTS

We found 41 upregulated and 19 downregulated differentially expressed (DE)-miRNAs in EVOO-EVs. Afterwards, SMCs were incubated with EVOO-EVs and SO-EVs. SMCs incubated with SO-EVs showed a greater number of DE-mRNA involved in pathways related to cancer, focal adhesion, regulation of actin cytoskeleton, and MAPK, toll-like receptor, chemokine and Wnt signaling pathways than in SMCs incubated with EVOO-EVs. These DE-mRNAs were involved in biological processes related to the response to endogenous stimulus, cell motility, regulation of intracellular signal transduction and cell population proliferation.

CONCLUSION

EVOO and SO can differently modify the miRNA composition of HUVEC-derived EVs. These EVs can regulate the SMCs transcriptomic profile, with SO-EVs promoting a profile more closely linked to the development of atherosclerosis than EVOO-EVs.

摘要

背景

关于特级初榨橄榄油（EVOO）和葵花籽油（SO）对内皮细胞分泌的细胞外囊泡（EVs）组成的影响以及这些EVs对平滑肌细胞（SMCs）的影响，目前了解甚少。这些细胞在动脉粥样硬化的发展中起重要作用。

方法

我们评估了用富含甘油三酯的脂蛋白（在高脂餐后获得）与EVOO（EVOO-EVs）和SO（SO-EVs）孵育后的内皮细胞衍生的EVs对SMCs转录组谱的影响。

结果

我们在EVOO-EVs中发现了41个上调和19个下调的差异表达（DE）-miRNA。之后，将SMCs与EVOO-EVs和SO-EVs孵育。与用EVOO-EVs孵育的SMCs相比，用SO-EVs孵育的SMCs显示出更多与癌症、粘着斑、肌动蛋白细胞骨架调节以及MAPK、Toll样受体、趋化因子和Wnt信号通路相关的DE-mRNA。这些DE-mRNA参与了与对内源性刺激的反应、细胞运动、细胞内信号转导调节和细胞群体增殖相关的生物学过程。

结论

EVOO和SO可以不同方式改变人脐静脉内皮细胞（HUVEC）衍生的EVs的miRNA组成。这些EVs可以调节SMCs的转录组谱，与EVOO-EVs相比，SO-EVs促进的转录组谱与动脉粥样硬化发展的联系更为紧密。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bf4/9039400/a57cbab9ba7d/fnut-09-867745-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bf4/9039400/e4460c30cde6/fnut-09-867745-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bf4/9039400/791c319318de/fnut-09-867745-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bf4/9039400/b9a2cc9e5d7c/fnut-09-867745-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bf4/9039400/a57cbab9ba7d/fnut-09-867745-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bf4/9039400/e4460c30cde6/fnut-09-867745-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bf4/9039400/791c319318de/fnut-09-867745-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bf4/9039400/b9a2cc9e5d7c/fnut-09-867745-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2bf4/9039400/a57cbab9ba7d/fnut-09-867745-g0004.jpg

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Endothelial Cell Receptors in Tissue Lipid Uptake and Metabolism.内皮细胞受体在组织脂质摄取和代谢中的作用。
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