Stephano-Hornedo José L, Torres-Gutiérrez Osmin, Toledano-Magaña Yanis, Gradilla-Martínez Israel, Pestryakov Alexey, Sánchez-González Alejandro, García-Ramos Juan Carlos, Bogdanchikova Nina
Meredith Gould Laboratories Tijuana Baja California Mexico.
Facultad de Ciencias, Universidad Autónoma de Baja California (UABC) Carretera Transpeninsular 3917 Ensenada Baja California 22860 Mexico.
RSC Adv. 2020 Feb 7;10(11):6146-6155. doi: 10.1039/c9ra09018e.
Nowadays, Huanglongbing (HLB) disease, commonly known as "yellow dragon disease", affects citrus crops worldwide and has a devastating effect in the agro-industrial sector. Significant efforts have been made to fight the illness, but still, there is no effective treatment to eradicate the disease. This work is the first approach to evaluate the capacity of silver nanoparticles (AgNPs) to directly eradicate the bacteria responsible for Huanglongbing disease, Liberibacter asiaticus (CLas), in the field. The AgNPs were administered by foliar sprinkling and trunk injection of 93 sick trees with remarkable results. Both methods produce an 80-90% decrease of bacterial titre, quantified by qRT-PCR in collected foliar tissue, compared with the control group. Scanning electron microscopy images show an essential reduction of starch accumulation in phloem vessels after AgNP treatments without evidence of bacteria in the analyzed samples. Compared with other effective methods that involve β-lactam antibiotics, the potency of AgNPs is 3 to 60-times higher when it is administered by foliar sprinkling and from 75 to 750-fold higher when the administration was by trunk-injection. All these results allow us to propose this AgNP formulation as a promising alternative for the treatment of infected trees in the field.
如今，黄龙病（HLB），俗称“黄梢病”，影响着全球的柑橘作物，给农业产业带来了毁灭性影响。人们已付出巨大努力来对抗这种疾病，但仍然没有根除该病的有效治疗方法。这项工作是首次评估银纳米颗粒（AgNPs）在田间直接根除引起黄龙病的细菌——亚洲韧皮杆菌（CLas）的能力。通过对93棵患病树木进行叶面喷洒和树干注射施用AgNPs，取得了显著效果。与对照组相比，两种方法均使收集的叶片组织中通过qRT-PCR定量的细菌滴度降低了80%-90%。扫描电子显微镜图像显示，AgNP处理后韧皮部导管中的淀粉积累显著减少，分析样品中未发现细菌迹象。与其他涉及β-内酰胺抗生素的有效方法相比，通过叶面喷洒施用时，AgNPs的效力高3至60倍，通过树干注射施用时，效力高75至750倍。所有这些结果使我们能够提出这种AgNP制剂作为田间感染树木治疗的一种有前景的替代方法。
