植物介导的银纳米粒子（AgNPs）增强抗氧化防御系统，改善 HLB 病‘Kinnow’蜜柑植株。

Phytomediated Silver Nanoparticles (AgNPs) Embellish Antioxidant Defense System, Ameliorating HLB-Diseased 'Kinnow' Mandarin Plants.

机构信息

Department of Botany, PMAS-Arid Agriculture University, Rawalpindi 46300, Pakistan.

Department of Plant Biology, Institute of Environmental Biology, Wrocław University of Environmental and Life Sciences, Kożuchowska 5b, 51-631 Wrocław, Poland.

出版信息

Molecules. 2023 Feb 22;28(5):2044. doi: 10.3390/molecules28052044.

DOI:10.3390/molecules28052044

PMID:36903290

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10004207/

Abstract

Citrus production is harmed worldwide by yellow dragon disease, also known as Huanglongbing (HLB), or citrus greening. As a result, it has negative effects and a significant impact on the agro-industrial sector. There is still no viable biocompatible treatment for Huanglongbing, despite enormous efforts to combat this disease and decrease its detrimental effects on citrus production. Nowadays, green-synthesized nanoparticles are gaining attention for their use in controlling various crop diseases. This research is the first scientific approach to examine the potential of phylogenic silver nanoparticles (AgNPs) to restore the health of Huanglongbing-diseased 'Kinnow' mandarin plants in a biocompatible manner. AgNPs were synthesized using as a reducing, capping, and stabilizing agent and characterized using different characterization techniques, i.e., UV-visible spectroscopy with a maximum average peak at 418 nm, scanning electron microscopy (SEM) with a size of 74 nm, and energy-dispersive spectroscopy (EDX), which confirmed the presence of silver ions along with different elements, and Fourier transform infrared spectroscopy served to confirm different functional groups of elements. Exogenously, AgNPs at various concentrations, i.e., 25, 50, 75, and 100 mgL, were applied against Huanglongbing-diseased plants to evaluate the physiological, biochemical, and fruit parameters. The findings of the current study revealed that 75 mgL AgNPs were most effective in boosting the plants' physiological profiles, i.e., chl a, chl b, total chl, carotenoid content, MSI, and RWC up to 92.87%, 93.36%, 66.72%, 80.95%, 59.61%, and 79.55%, respectively; biochemical parameters, i.e., 75 mgL concentration decreased the proline content by up to 40.98%, and increased the SSC, SOD, POD, CAT, TPC, and TFC content by 74.75%, 72.86%, 93.76%, 76.41%, 73.98%, and 92.85%, respectively; and fruit parameters, i.e., 75 mgL concentration increased the average fruit weight, peel diameter, peel weight, juice weight, rag weight, juice pH, total soluble solids, and total sugarby up to 90.78%, 8.65%, 68.06%, 84.74%, 74.66%, 52.58%, 72.94%, and 69.69%, respectively. These findings enable us to develop the AgNP formulation as a potential citrus Huanglongbing disease management method.

摘要

柑橘生产受到黄龙病（也称为黄龙病或柑橘绿化病）的全球危害，这对农业产业部门产生了负面影响和重大影响。尽管为防治这种疾病及其对柑橘生产的不利影响作出了巨大努力，但仍没有可行的生物相容性治疗方法。如今，绿色合成纳米粒子因其在控制各种作物疾病方面的用途而受到关注。本研究首次采用系统发育银纳米粒子（AgNPs）以生物相容性方式恢复黄龙病患病‘金诺’柑橘植物健康的科学方法。AgNPs 是使用作为还原剂、封端剂和稳定剂合成的，并使用不同的表征技术进行了表征，即最大平均峰在 418nm 的紫外-可见光谱、尺寸为 74nm 的扫描电子显微镜（SEM）和能量色散光谱（EDX），证实了银离子的存在以及不同的元素，傅立叶变换红外光谱用于确认元素的不同官能团。AgNPs 以不同浓度，即 25、50、75 和 100mgL，外源施用于黄龙病患病植物以评估生理、生化和果实参数。目前研究的结果表明，75mgL 的 AgNPs 最有效地提高了植物的生理特性，即叶绿素 a、叶绿素 b、总叶绿素、类胡萝卜素含量、MSI 和 RWC 至 92.87%、93.36%、66.72%、80.95%、59.61%和 79.55%；生化参数，即 75mgL 浓度降低了脯氨酸含量高达 40.98%，并增加了 SSC、SOD、POD、CAT、TPC 和 TFC 含量达 74.75%、72.86%、93.76%、76.41%、73.98%和 92.85%；以及果实参数，即 75mgL 浓度提高了平均果实重量、果皮直径、果皮重量、果汁重量、瓤重量、果汁 pH 值、总可溶性固形物和总糖含量高达 90.78%、8.65%、68.06%、84.74%、74.66%、52.58%、72.94%和 69.69%。这些发现使我们能够开发 AgNP 配方作为柑橘黄龙病管理的潜在方法。