Pérez J L, Berrocal C I, Berrocal L
J Appl Bacteriol. 1986 Dec;61(6):541-5. doi: 10.1111/j.1365-2672.1986.tb01727.x.
A commercial beta-glucuronidase (beta-GUR) test for the rapid and economical identification of Escherichia coli was evaluated. A total of 762 clinical strains and 228 environmental isolates were studied. More than 95% of the E. coli strains were found to be beta-GUR positive. Thirty-one clinical isolates of Shigella sonnei, 10 of Enterobacter cloacae, eight of Enterobacter aerogenes, nine of Citrobacter freundii and one of Salmonella enteritidis also gave positive results. The enzyme beta-GUR was also detected in two environmental strains of E. cloacae and one C. freundii. A comparative study between the beta-GUR test and the conventional identification system was carried out in 233 consecutive isolates of lactose positive enterobacteria. Agreement was observed in 223 cases and 190 E. coli strains were correctly identified using this test. Discrepancies were found in 10 cases: nine E. coli were beta-GUR negative and one C. freundii was beta-GUR positive. Escherichia coli was the only species positive for both beta-GUR and indole tests. This procedure permits a rapid, easy, precise and inexpensive identification of E. coli. beta-GUR positive Enterobacter strains have not previously been described.
对一种用于快速且经济地鉴定大肠杆菌的商业β-葡萄糖醛酸酶（β-GUR）检测方法进行了评估。共研究了762株临床菌株和228株环境分离株。发现超过95%的大肠杆菌菌株β-GUR呈阳性。31株宋内志贺菌临床分离株、10株阴沟肠杆菌、8株产气肠杆菌、9株弗氏柠檬酸杆菌和1株肠炎沙门氏菌也呈阳性结果。在两株阴沟肠杆菌环境菌株和一株弗氏柠檬酸杆菌中也检测到了β-GUR酶。对233株连续的乳糖阳性肠杆菌分离株进行了β-GUR检测与传统鉴定系统之间的比较研究。在223例中观察到一致性，使用该检测方法正确鉴定出190株大肠杆菌菌株。在10例中发现了差异：9株大肠杆菌β-GUR呈阴性，1株弗氏柠檬酸杆菌β-GUR呈阳性。大肠杆菌是唯一β-GUR和吲哚检测均为阳性的菌种。该方法可快速、简便、准确且廉价地鉴定大肠杆菌。此前尚未描述过β-GUR阳性的肠杆菌菌株。
