Tharushi Perera Palalle G, Todorova Nevena, Vilagosh Zoltan, Bazaka Olha, Nguyen The Hong Phong, Bazaka Kateryna, Crawford Russell J, Croft Rodney J, Yarovsky Irene, Ivanova Elena P
School of Science, RMIT University PO Box 2476 Melbourne VIC 3001 Australia
Faculty Science, Engineering and Technology, Swinburne University of Technology PO Box 218 Hawthorn VIC 3122 Australia.
RSC Adv. 2021 Sep 23;11(50):31408-31420. doi: 10.1039/d1ra05459g. eCollection 2021 Sep 21.
Membrane model systems capable of mimicking live cell membranes were used for the first time in studying the effects arising from electromagnetic fields (EMFs) of 18 GHz where membrane permeability was observed following exposure. A present lack of understanding of the mechanisms that drive such a rapid change in membrane permeabilization as well as any structural or dynamic changes imparted on biomolecules affected by high-frequency electromagnetic irradiation limits the use of 18 GHz EMFs in biomedical applications. A phospholipid, 1,2-dioleoyl--glycero-3-phosphocholine (DOPC) labelled with a fluorescent marker 1,2-dioleoyl--glycero-3-phosphoethanolamine--(lissamine rhodamine B sulfonyl) (rhodamine-DOPE) was used in constructing the giant unilamellar vesicles (GUVs). After three cycles of exposure, enhanced membrane permeability was observed by the internalisation of hydrophilic silica nanospheres of 23.5 nm and their clusters. All-atom molecular dynamics simulations of 1-palmitoyl-2-oleoyl--glycero-3-phosphocholine (POPC) membranes exposed to high frequency electric fields of different field strengths showed that within the simulation timeframe only extremely high strength fields were able to cause an increase in the interfacial water dynamics characterized by water dipole realignments. However, a lower strength, high frequency EMF induced changes of the water hydrogen bond network, which may contribute to the mechanisms that facilitate membrane permeabilization in a longer timeframe.
首次使用能够模拟活细胞膜的膜模型系统来研究18 GHz电磁场（EMF）产生的影响，其中在暴露后观察膜通透性。目前对驱动膜通透性如此快速变化的机制以及受高频电磁辐射影响的生物分子所产生的任何结构或动态变化缺乏了解，这限制了18 GHz EMF在生物医学应用中的使用。一种用荧光标记物1,2 - 二油酰基 - 甘油 - 3 - 磷酸乙醇胺 - （丽丝胺罗丹明B磺酰基）（罗丹明 - DOPE）标记的磷脂1,2 - 二油酰基 - 甘油 - 3 - 磷酸胆碱（DOPC）被用于构建巨型单层囊泡（GUV）。经过三个暴露周期后，通过23.5 nm亲水性二氧化硅纳米球及其聚集体的内化观察到膜通透性增强。对暴露于不同场强高频电场的1 - 棕榈酰基 - 2 - 油酰基 - 甘油 - 3 - 磷酸胆碱（POPC）膜进行的全原子分子动力学模拟表明，在模拟时间范围内，只有极高强度的场能够导致以水偶极重新排列为特征的界面水动力学增加。然而，较低强度的高频EMF会引起水氢键网络的变化，这可能有助于在更长时间范围内促进膜通透性的机制。
