Liu Haifeng, Zhu Xinyue, Dong Qinting, Qiao Chengpeng, Luo Yuhang, Liu Yi, Zou Yanlin, Liu Huanghao, Wu Cuilan, Su Jieyu, Peng Hao, Ouyang Kang, Chen Ying, Li Jun, Wei Zuzhang, Huang Weijian
Laboratory of Animal Infectious Diseases and Molecular Immunology, College of Animal Science and Technology, Guangxi University, Nanning, China.
Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning, China.
Front Vet Sci. 2022 Apr 14;9:851743. doi: 10.3389/fvets.2022.851743. eCollection 2022.
In recent years, hunniviruses have been reported in a variety of animal species from many countries. Here, hunnivirus was detected in fecal samples from water buffaloes and named as BufHuV-GX-2106. The samples were inoculated into cultures of MDBK cells supplemented with TPCK trypsin and the BufHuV-GX-2106 strain was stably passaged and replicated. Electron microscopic analysis showed the BufHuV-GX-2106 virus particles were spherical and 20~30 nm in diameter. The complete genome of a plaque purified sample of BufHuV-GX-2106 was determined and analyzed. Genomic analysis revealed that the whole sequence of BufHuV-GX-2106 was ~7,601 nucleotides (nt) in length and consisted of a large open reading frame of 6,759nt, a 5'UTR, a 3'UTR and a poly(A) tail. The complete genome sequence of BufHuV-GX-2106 shares 68-85% nucleotide identities with other known hunnivirus strains, indicating high genetic heterogeneity among these viruses. Phylogenetic analysis showed that BufHuV-GX-2106 belonged to the species and was more closely related to ovine hunnivirus than other known viruses of this type. This study describes the first isolation and complete genome sequence of a hunnivirus strain from water buffaloes. In addition, this study will help to understand the mechanisms involved in the pathogenesis of among different animal species.
近年来，许多国家的多种动物物种中都报告发现了亨尼病毒。在此，从水牛粪便样本中检测到了亨尼病毒，并将其命名为BufHuV-GX-2106。将样本接种到添加了TPCK胰蛋白酶的MDBK细胞培养物中，BufHuV-GX-2106毒株得以稳定传代和复制。电子显微镜分析显示，BufHuV-GX-2106病毒颗粒呈球形，直径为20至30纳米。对BufHuV-GX-2106的一个蚀斑纯化样本的完整基因组进行了测定和分析。基因组分析表明，BufHuV-GX-2106的全序列长度约为7601个核苷酸（nt），由一个6759nt的大开放阅读框、一个5'UTR、一个3'UTR和一个聚腺苷酸尾巴组成。BufHuV-GX-2106的完整基因组序列与其他已知亨尼病毒毒株的核苷酸同一性为68%-85%，表明这些病毒之间存在高度的遗传异质性。系统发育分析表明，BufHuV-GX-2106属于该物种，与绵羊亨尼病毒的亲缘关系比其他已知此类病毒更为密切。本研究描述了从水牛中分离出的亨尼病毒毒株的首次分离及完整基因组序列。此外，本研究将有助于了解不同动物物种中[此处原文缺失相关疾病名称]发病机制所涉及的机制。
Zotero 插件
