叶多糖对环磷酰胺处理小鼠免疫反应和肠道微生物群组成的调节作用

leaf polysaccharides regulation of immune response and gut microbiota composition in cyclophosphamide-treated mice.

作者信息

Xie Hualing, Fang Jingyu, Farag Mohamed A, Li Zhenhao, Sun Peilong, Shao Ping

机构信息

College of Food Science and Technology, Zhejiang University of Technology, Zhejiang, Hangzhou 310014, PR China.

Pharmacognosy Department, College of Pharmacy, Cairo University, Kasr El Aini St. P.B, Cairo, Egypt.

出版信息

Food Chem X. 2022 Jan 29;13:100235. doi: 10.1016/j.fochx.2022.100235. eCollection 2022 Mar 30.

In this study, the polysaccharides extracted from leaf (DOLP) was used in immune deficiency mice to evaluate the bioactivity. Thymus and spleen indices were calculated while the alleviation of the colon and liver histopathological progression was evaluated by H&E staining. The data indicated that DOLP improved immunity status by restoring the gut barrier and atrophy of immune organs. Cytokines levels as marker of inflammation were determined using ELISA in serum and colon. Which proved that DOLP inhibited the expression of pro-inflammatory cytokines (TNF-, TGF- 1, IL-6, IL-1) and promoted the expression of anti-inflammatory cytokines (IL-10). Short chain fatty acids (SCFAs) levels and microbial composition in feces were determined using GS and high-throughput sequencing. DOLP improved gut microbiota by increasing the relative abundance of total bacteria and probiotics such as , and Therefore, DOLP has potential effect for the treatment of chronic immune diseases.

在本研究中，从叶片中提取的多糖（DOLP）被用于免疫缺陷小鼠以评估其生物活性。计算胸腺和脾脏指数，同时通过苏木精-伊红（H&E）染色评估结肠和肝脏组织病理学进展的缓解情况。数据表明，DOLP通过恢复肠道屏障和免疫器官萎缩来改善免疫状态。使用酶联免疫吸附测定（ELISA）法测定血清和结肠中作为炎症标志物的细胞因子水平。这证明DOLP抑制促炎细胞因子（TNF-、TGF-1、IL-6、IL-1）的表达并促进抗炎细胞因子（IL-10）的表达。使用气相色谱（GS）和高通量测序法测定粪便中的短链脂肪酸（SCFAs）水平和微生物组成。DOLP通过增加总细菌和益生菌（如、和）的相对丰度来改善肠道微生物群。因此，DOLP对慢性免疫疾病具有潜在的治疗作用。