Department of Orthodontics, Dental School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Orthodontics, Dental School, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Am J Orthod Dentofacial Orthop. 2022 Jul;162(1):e17-e27. doi: 10.1016/j.ajodo.2021.11.014. Epub 2022 Apr 29.
Bisphosphonates can severely slow down orthodontic tooth movement (OTM) by reducing bone turnover. This calls for materials and methods to reverse or neutralize their effects on OTM. We propose systemic vitamin D3 (D3) for this purpose.
Thirty-two male Wistar rats were randomized into 4 groups of 8 each. Three groups were administered D3 (3 systemic doses of 24,000 IU/kg each), alendronate (ALN) (5 doses of 7 mg/kg each), and ALN+D3 (same doses as mentioned above). One group served as the negative control. The incisors were distalized at 30 g of force for 2 weeks. OTMs were measured blindly. Radicular pressure areas were searched histologically (blindly) for capillaries, Howship's lacunae, osteoclasts, and osteoblasts. Data were analyzed statistically (α = 0.05, α = 0.0083, β <0.1).
OTMs in the groups D3, ALN+D3, ALN, and control were 1.900 ± 0.237, 1.629 ± 0.219, 0.975 ± 0.145, and 1.565 ± 0.324 mm (analysis of variance, P <0.001), respectively. OTM in the ALN group was smaller than all other groups (Tukey, P <0.001). OTM in the D3 group was greater than in the control group (P = 0.054). The ALN+D3 group had greater OTM than the ALN group (P <0.001) but was not significantly different from the D3 (P = 0.153) or control (P = 0.951) groups. All histologic variables were significantly different across groups (Kruskal-Wallis, P <0.001). All the markers in the D3 group were more frequent than those of the other groups (Mann-Whitney U, P <0.001). There were fewer markers in the ALN group than in the control group (P ≤0.001). The ALN+D3 group had more markers than the ALN group in terms of capillaries, osteoclasts, and osteoblasts (P ≤0.007). The ALN+D3 group was similar to the control group regarding capillaries, osteoclasts, and osteoblasts (P ≥0.382).
Systemic vitamin D3 may accelerate OTM and increase histologic biomarkers of bone turnover. ALN reduces OTM and its histologic biomarkers. Systemic vitamin D3 can reverse this inhibitory effect of ALN on OTM back to normal.
双膦酸盐可通过减少骨转换，严重减缓正畸牙齿移动（OTM）。这需要寻找材料和方法来逆转或中和它们对 OTM 的影响。我们为此提出了全身性维生素 D3（D3）。
将 32 只雄性 Wistar 大鼠随机分为 4 组，每组 8 只。三组给予 D3（3 次全身剂量 24000 IU/kg ），阿仑膦酸盐（ALN）（5 次剂量 7mg/kg ）和 ALN+D3（如上所述的相同剂量）。一组作为阴性对照。将切牙以 30g 的力远移 2 周。盲法测量 OTM。盲法搜索根面压力区的毛细血管、豪氏陷窝、破骨细胞和成骨细胞。数据进行统计学分析（α=0.05，α=0.0083，β<0.1）。
D3 组、ALN+D3 组、ALN 组和对照组的 OTM 分别为 1.900±0.237、1.629±0.219、0.975±0.145 和 1.565±0.324mm（方差分析，P<0.001）。ALN 组的 OTM 小于其他所有组（Tukey，P<0.001）。D3 组的 OTM 大于对照组（P=0.054）。ALN+D3 组的 OTM 大于 ALN 组（P<0.001），但与 D3 组（P=0.153）或对照组（P=0.951）无显著差异。各组间所有组织学变量均有显著差异（Kruskal-Wallis，P<0.001）。D3 组的所有标志物均比其他组更频繁（Mann-Whitney U，P<0.001）。ALN 组的标志物少于对照组（P≤0.001）。ALN+D3 组在毛细血管、破骨细胞和成骨细胞方面的标志物多于 ALN 组（P≤0.007）。ALN+D3 组与对照组在毛细血管、破骨细胞和成骨细胞方面相似（P≥0.382）。
全身性维生素 D3 可能加速 OTM 并增加骨转换的组织学生物标志物。ALN 减少 OTM 及其组织学生物标志物。全身性维生素 D3 可以逆转 ALN 对 OTM 的抑制作用，使其恢复正常。
