Jang Woong Sik, Lee Jun Min, Lee Eunji, Park Seoyeon, Lim Chae Seung
Emergency Medicine, College of Medicine, Korea University Guro Hospital, 148, Gurodong-ro, Guro-gu, Seoul 08308, Republic of Korea.
BK21 Graduate Program, Department of Biomedical Sciences, College of Medicine, Korea University, 145 Anam-ro, Seongbuk-gu, Seoul 02841, Republic of Korea.
Diagnostics (Basel). 2024 May 6;14(9):967. doi: 10.3390/diagnostics14090967.
Influenza viruses cause highly contagious respiratory diseases that cause millions of deaths worldwide. Rapid detection of influenza viruses is essential for accurate diagnosis and the initiation of appropriate treatment. We developed a loop-mediated isothermal amplification and lateral flow assay (LAMP-LFA) capable of simultaneously detecting influenza A and influenza B. Primer sets for influenza A and influenza B were designed to target conserved regions of segment 7 and the nucleoprotein gene, respectively. Optimized through various primer set ratios, the assay operated at 62 °C for 30 min. For a total of 243 (85 influenza A positive, 58 influenza B positive and 100 negative) nasopharyngeal swab samples, the performance of the influenza A/B multiplex LAMP-LFA was compared with that of the commercial Allplex Respiratory Panel 1 assay (Seegene, Seoul, Korea). The influenza A/B multiplex LAMP-LFA demonstrated a specificity of 98% for the non-infected clinical samples, along with sensitivities of 94.1% for the influenza A clinical samples and 96.6% for the influenza B clinical samples, respectively. The influenza A/B multiplex LAMP-LFA showed high sensitivity and specificity, indicating that it is reliable for use in a low-resource environment.
流感病毒会引发具有高度传染性的呼吸道疾病，在全球范围内导致数百万人死亡。快速检测流感病毒对于准确诊断和启动适当治疗至关重要。我们开发了一种能够同时检测甲型流感和乙型流感的环介导等温扩增和侧向流动分析方法（LAMP-LFA）。针对甲型流感和乙型流感的引物组分别设计为靶向第7节段的保守区域和核蛋白基因。通过各种引物组比例进行优化后，该分析方法在62°C下运行30分钟。对于总共243份（85份甲型流感阳性、58份乙型流感阳性和100份阴性）鼻咽拭子样本，将甲型/乙型流感多重LAMP-LFA的性能与商用Allplex Respiratory Panel 1分析方法（韩国首尔Seegene公司）进行了比较。甲型/乙型流感多重LAMP-LFA对未感染的临床样本显示出98%的特异性，对甲型流感临床样本和乙型流感临床样本的敏感性分别为94.1%和96.6%。甲型/乙型流感多重LAMP-LFA显示出高敏感性和特异性，表明其在资源匮乏的环境中使用是可靠的。
