泛菌属微囊藻新种，从富营养化水库中在微囊藻水华期间分离得到。

Panacibacter microcysteis sp. nov., isolated from a eutrophic reservoir during the Microcystis bloom period.

机构信息

Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.

Department of Environmental Biotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), 217 Gajeong-ro, Yuseong-gu, Daejeon, 34113, Republic of Korea.

出版信息

Arch Microbiol. 2022 May 3;204(6):291. doi: 10.1007/s00203-022-02893-y.

DOI:10.1007/s00203-022-02893-y

PMID:35503579

Abstract

A Gram-stain-negative, rod-shaped bacterial strain DH6 was isolated from fresh water of the Daechung Reservoir during the Microcystis bloom period. The strain grew at pH 6.0-8.5, at temperature 17-40 °C, and at 0-1% (w/v) NaCl concentration. Comparison of 16S rRNA gene sequence indicated that strain DH6 exhibits the highest similarity with Panacibacter ginsenosidivorans Gsoil 1550 (96.6%). The average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) values of strain DH6 compared to its related type strains were below 74.2%, 22.3%, and 74.8%, respectively. The predominant fatty acids (> 5.0%) were identified as iso-C 3-OH, iso-C, iso-C, C 2-OH, iso-C, anteiso-C, and iso-C G. The polar lipid profile contained phosphatidylethanolamine, four unidentified aminolipids, and three unidentified lipids. The respiratory quinone was menaquinone 7 (MK-7). The genomic DNA G + C content was 42.6%. Collectively, strain DH6 should be classified as a novel species within the genus Panacibacter, for which the name Panacibacter microcysteis sp. nov. is proposed. The type strain is DH6 (= KCTC 82471 = LMG 32426).

摘要

一株革兰氏阴性、杆状的细菌菌株 DH6 是在微囊藻水华期间从大清水库的淡水环境中分离得到的。该菌株在 pH 值 6.0-8.5、温度 17-40°C 和 0-1%（w/v）NaCl 浓度的条件下生长。16S rRNA 基因序列比较表明，菌株 DH6 与 Panacibacter ginsenosidivorans Gsoil 1550（96.6%）具有最高的相似性。与相关模式菌株相比，菌株 DH6 的平均核苷酸同一性（ANI）、数字 DNA-DNA 杂交（dDDH）和平均氨基酸同一性（AAI）值分别低于 74.2%、22.3%和 74.8%。主要脂肪酸（>5.0%）为 iso-C3-OH、iso-C、iso-C、C2-OH、iso-C、anteiso-C 和 iso-C G。极性脂谱包含磷脂酰乙醇胺、四种未鉴定的氨基脂和三种未鉴定的脂类。呼吸醌为menaquinone 7（MK-7）。基因组 DNA G+C 含量为 42.6%。综合来看，菌株 DH6 应归类为 Panacibacter 属中的一个新种，命名为 Panacibacter microcysteis sp. nov.。其模式菌株为 DH6（=KCTC 82471=LMG 32426）。