Cell Factory Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.
Department of Environmental Biotechnology, KRIBB School of Biotechnology, University of Science and Technology (UST), Daejeon 34113, Republic of Korea.
J Microbiol Biotechnol. 2022 Dec 28;32(12):1553-1560. doi: 10.4014/jmb.2208.08021. Epub 2022 Oct 31.
A Gram-stain-negative, rod-shaped bacterial strain, JC4, was isolated from a freshwater sample and determined the taxonomic position. Initial identification based on 16S rRNA gene sequences revealed that strain JC4 is affiliated to the genus with a sequence similarity of 97.97% to WPCB133. The average nucleotide identity and digital DNA-DNA hybridization values between strain JC4 and species were estimated below 80.92% and 23.9%, respectively. Strain JC4 contained summed feature 3 (C 7 and/or C 6) and iso-C as predominant cellular fatty acids. The dominant polar lipids were identified as phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified phospholipid, and two unidentified lipids. The respiratory quinone was MK-7. The genomic DNA G+C content of strain JC4 was determined to be 42.44%. The above polyphasic evidences support that strain JC4 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is JC4 (= KCTC 92230 = LMG 32715).
一株革兰氏阴性、杆状的细菌 JC4 菌株，从淡水样本中分离出来，并确定了其分类地位。基于 16S rRNA 基因序列的初步鉴定表明，菌株 JC4 属于 属，与 WPCB133 的序列相似性为 97.97%。菌株 JC4 与 种之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别估计为低于 80.92%和 23.9%。菌株 JC4 含有主要的细胞脂肪酸为(summed feature 3，C7 和/或 C6)和 iso-C。主要的极性脂类被鉴定为磷脂酰乙醇胺、一种未鉴定的氨基磷脂、一种未鉴定的磷脂和两种未鉴定的脂类。呼吸醌为 MK-7。菌株 JC4 的基因组 DNA G+C 含量为 42.44%。上述多相证据支持菌株 JC4 代表了 属的一个新种，因此提议使用新种名 sp. nov.。模式菌株为 JC4(=KCTC 92230=LMG 32715)。
