Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Sumatera Utara, Medan, 20155, Indonesia.
Department of Agroecotechnology, Faculty of Agriculture, Universitas Sumatera Utara, Medan, 20155, Indonesia.
F1000Res. 2021 Sep 15;10:923. doi: 10.12688/f1000research.72982.2. eCollection 2021.
Binahong ( (Ten.) STEENIS) is a widely available herbal plant in Indonesia and has been intensely researched for its healing abilities due to its biological activities, but few have studied its capability in accelerating hard tissue healing in post-extraction tooth sockets. The purpose of this study was to analyse the effects of 3% binahong leaf extract gel on alveolar bone healing in post-extraction sockets in Wistar rats. In this study, 48 male Wistar rats were randomly allocated to twelve groups. After the extraction of the left mandibular incisor, sockets in Group I to IV were given 3% binahong leaf extract gel, group V to VIII were given a control gel, and group IX to XII were given Gengigel for 14 days. The residual socket volume (RSV) and fibroblast proliferation were observed on the 3 , 7 , and 14 day post-extraction, while the osteoblast and osteocyte proliferation were observed on the 7 , 14 , and 28 day post-extraction. The RSV data were analysed using repeated measure ANOVA and one-way ANOVA, while the histopathological data were analysed using one-way ANOVA. The results showed that the binahong group had the lowest RSV and the highest fibroblast proliferation compared to the other groups on the 7th day (p<0.05) and the highest osteoblast and osteocyte proliferation compared to the other groups on the 14 day (p<0.05). The experiment showed that 3% binahong leaf extract gel could accelerate wound closure, which was characterized by a greater decrease in the RSV value in comparison to the other treatment groups and could enhance alveolar bone healing by increasing the proliferation of fibroblasts, osteoblasts, and osteocytes.
binahong ( (Ten.) STEENIS) 是印度尼西亚一种广泛应用的草药植物，由于其生物活性，它的治疗能力已经得到了深入的研究，但很少有人研究它在加速拔牙后牙槽骨愈合方面的能力。本研究旨在分析 3%binahong 叶提取物凝胶对 Wistar 大鼠拔牙后牙槽骨愈合的影响。在这项研究中，48 只雄性 Wistar 大鼠被随机分为 12 组。在拔除左侧下颌切牙后，第 I 组至第 IV 组的牙槽窝给予 3%binahong 叶提取物凝胶，第 V 组至第 VIII 组给予对照凝胶，第 IX 组至第 XII 组给予 Gengigel 凝胶，共 14 天。在拔牙后第 3、7 和 14 天观察剩余牙槽窝体积（RSV）和纤维母细胞增殖，在拔牙后第 7、14 和 28 天观察成骨细胞和骨细胞增殖。RSV 数据采用重复测量方差分析和单因素方差分析进行分析，组织病理学数据采用单因素方差分析进行分析。结果表明，与其他组相比，binahong 组在第 7 天（p<0.05）的 RSV 最低，纤维母细胞增殖最高，在第 14 天（p<0.05）的成骨细胞和骨细胞增殖最高。实验表明，3%binahong 叶提取物凝胶能加速伤口闭合，与其他治疗组相比，RSV 值下降幅度更大，能通过增加纤维母细胞、成骨细胞和骨细胞的增殖来增强牙槽骨愈合。
