Yuniarti Wiwik Misaco, Lukiswanto Bambang Sektiari
Department of Clinical Science, Faculty of Veterinary Medicine, Universitas Airlangga, Mulyorejo, Kampus C Unair, Surabaya 60115, Indonesia.
Vet World. 2017 Jul;10(7):808-813. doi: 10.14202/vetworld.2017.808-813. Epub 2017 Jul 22.
Skin burn is a health problem that requires fast and accurate treatment. If not well-treated, the burn will cause various damaging conditions for the patient. The leaf extract of Madeira vine ( (Ten.) Steenis), or popularly known as Binahong in Indonesia, has been used to treat various diseases. The purpose of this research is to determine the effects of leaf extracts of Madeira vine ( (Ten.) Steenis) on skin burn healing process in rats as an animal model.
In this research, there were four treatment groups: G0, G1, G2, and G3, each consisting of five rats. All these rats were given skin burns, using hot metal plates. Then, sulfadiazine was given to G0, 2.5% leaf extract of Madeira vine was given to G1, 5% extract was given to G2, and 10% extract was given to G3, for straight 14 days topically, 3 times a day. At the end of the treatment period, skin excisions were conducted, and histopathological examination was carried out.
Microscopic observation on the wound healing process on the collagen deposition, polymorphonuclear infiltration, angiogenesis, and fibrosis showed that G2 had a significant difference with G0, G1, and G3 (p<0.05), while group G0 was significantly different from G1 and G3 (p<0.05). The better burn healing process on G2 allegedly because of the activity of flavonoid, saponin, and tannin, contained in the Madeira vine, which have the antioxidant, anti-inflammatory, and antibacterial effects.
The ointment from the 5% leaf extract of Madeira vine ( (Ten.) Steenis) has been proven to be effective to be used for topical burn therapy.
皮肤烧伤是一个需要快速准确治疗的健康问题。如果治疗不当，烧伤会给患者带来各种损害。藤三七（(Ten.) Steenis）的叶提取物，在印度尼西亚俗称“Binahong”，已被用于治疗多种疾病。本研究的目的是确定藤三七（(Ten.) Steenis）叶提取物对大鼠皮肤烧伤愈合过程的影响，以大鼠作为动物模型。
本研究有四个治疗组：G0、G1、G2和G3，每组由五只大鼠组成。所有这些大鼠均使用热金属板造成皮肤烧伤。然后，G0给予磺胺嘧啶，G1给予2.5%的藤三七叶提取物，G2给予5%的提取物，G3给予10%的提取物，连续14天局部给药，每天3次。在治疗期结束时，进行皮肤切除，并进行组织病理学检查。
对伤口愈合过程中胶原沉积、多形核细胞浸润、血管生成和纤维化的显微镜观察表明，G2与G0、G1和G3有显著差异（p<0.05），而G0组与G1和G3有显著差异（p<0.05）。据称G2的烧伤愈合过程更好是因为藤三七中含有的黄酮类、皂苷和单宁的活性，它们具有抗氧化、抗炎和抗菌作用。
藤三七（(Ten.) Steenis）5%叶提取物制成的软膏已被证明可有效用于局部烧伤治疗。
