Kusriani Herni, Susilawati Elis, Nurafipah Lytia
Department of Biology Pharmacy, Faculty of Pharmacy, Bhakti Kencana University, Bandung, Indonesia.
J Pharm Bioallied Sci. 2023 Apr-Jun;15(2):75-80. doi: 10.4103/jpbs.jpbs_917_21. Epub 2023 Jun 8.
Diabetes mellitus (DM) is a group of metabolic disorders characterized by hyperglycemia. Diabetes mellitus is a silent killer because sufferers are often not aware of it when it is realized, complications have occurred. Treatment for this disease must be done for life to control blood sugar in the body; however, oral antidiabetic drugs often produce unwanted side effects such as bloating, diarrhea, and stomach cramps. One of the treatments for diabetes is to find sources of treatment using natural ingredients that are relatively safe, including using plants as medicines. Based on several studies, Binahong leaves ( Ten. Steenis), brotowali ( L.), and cherry (.) are medicinal plants that can be used to reduce blood sugar levels. This study aims to test the antidiabetic activity using and testing methods of extracts of binahong leaves, cherry leaves, brotowali stems and their combinations.
method uses animal modeling of insulin deficiency, whereas method with alpha glycosidase inhibition activity assay. The administration of extracts was repeated every day for 14 days and blood glucose levels were measured on the 0, 7, and 14 days. Then surgery was performed on the pancreas and calculated the area of the islets of Langerhans, and the number of alpha and beta cells in the pancreas. The inhibitory activity of the alpha-glucosidase enzyme with the IC value of each extract and its combination was determined, with acarbose used as a standard.
The combination of binahong leaves ( Ten.Steenis) and cherry leaves ( L.) and the combination of brotowali stems ( (L.) and binahong leaves showed antidiabetic activity with insulin deficiency method. The combination of these extracts was able to reduce blood sugar levels until the observation on day 14. In testing by inhibiting alpha-glucosidase enzymes, binahong leaves extract, brotowali stems, and cherry leaves were able to inhibit alpha-glucosidase enzymes at IC of 35.07 ± 2.35; 29.42 ± 1.40; and 26.63 ± 1.15, respectively.
The best combination of extracts by and methods was shown in the combination of binahong leaf and brotowal stem extract binahong leaves, brotowali stems (2:1).
糖尿病（DM）是一组以高血糖为特征的代谢紊乱疾病。糖尿病是一种无声的杀手，因为患者在发病时往往没有意识到，此时并发症已经发生。这种疾病的治疗必须终身进行以控制体内血糖；然而，口服抗糖尿病药物常常会产生不良副作用，如腹胀、腹泻和胃痉挛。糖尿病的治疗方法之一是寻找相对安全的天然成分治疗来源，包括使用植物作为药物。基于多项研究，红凤菜叶（Ten. Steenis）、布罗托瓦利（L.）和樱桃（.）是可用于降低血糖水平的药用植物。本研究旨在通过红凤菜叶、樱桃叶、布罗托瓦利茎提取物及其组合的实验和测试方法来检测其抗糖尿病活性。
实验方法采用胰岛素缺乏动物模型，而测试方法采用α-葡萄糖苷酶抑制活性测定。提取物每天重复给药14天，并在第0、7和14天测量血糖水平。然后对胰腺进行手术，计算胰岛面积以及胰腺中α细胞和β细胞的数量。以阿卡波糖为标准，测定各提取物及其组合对α-葡萄糖苷酶的抑制活性及IC值。
红凤菜叶（Ten.Steenis）和樱桃叶（L.）的组合以及布罗托瓦利茎（(L.)）和红凤菜叶的组合在胰岛素缺乏实验方法中显示出抗糖尿病活性。这些提取物的组合能够将血糖水平降低至第14天的观察值。在通过抑制α-葡萄糖苷酶进行的测试中，红凤菜叶提取物、布罗托瓦利茎和樱桃叶分别能够在IC值为35.07±2.35；29.42±1.40；和26.63±1.15时抑制α-葡萄糖苷酶。
通过实验和测试方法得出的最佳提取物组合是红凤菜叶和布罗托瓦利茎提取物的组合（红凤菜叶、布罗托瓦利茎，2:1）。
