iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Av. Rovisco Pais, 1049-001 Lisboa, Portugal.
Int J Syst Evol Microbiol. 2022 May;72(5). doi: 10.1099/ijsem.0.005388.
The species Sesma and Ramirez was delineated based on the description of the single strain CBS 139.77. Based on its phenotypic similarities to , CBS 139.77 was later considered a synonym of . In the present study, we isolated the yeast strain IST 508 (=PYCC 8784=CBS 16671) from the soil surrounding an olive tree in Ferreira do Alentejo, Portugal. The phylogenetic analysis of D1/D2 domain and ITS sequences from strain IST 508 indicates that is closely related to . Although strain IST 508 differs from CBS 139.77 by 14 substitutions and 20 indels (6.6 % divergence) in the ITS sequence, no divergence was detected at the level of D1/D2 domain, mitochondrial small subunit rDNA, and cytochrome oxidase II sequences. On the other hand, strains IST 508 and CBS 139.77 differ from NRRL Y-17577 by eight substitutions (1.4 % divergence) in the D1/D2 domain sequence, by 16 substitutions (2.7 % divergence) in the cytochrome oxidase II sequence, and by 16 substitutions (3.7 % divergence) in the mitochondrial small subunit rDNA sequence. Due to the high number of variable phenotypic tests in and , strains from the two species are difficult to distinguish. Contrasting with what is described for other species, no differences were detected at the level of micromorphology between the two species. Nevertheless, based on the molecular differences between the two strains, CBS 139.77 and IST 508, and NRRL Y-17577 and their phylogenetic analysis, strains CBS 139.77 and IST 508 are from and this species should be considered as an independent species and not a later synonym of . We propose an emended description of .
Sesma 和 Ramirez 这两个种是基于单一菌株 CBS 139.77 的描述而划定的。基于其与的表型相似性，CBS 139.77 后来被认为是的同义词。在本研究中，我们从葡萄牙费雷拉-多-阿尔entejo 的一棵橄榄树周围的土壤中分离出酵母菌株 IST 508（=PYCC 8784=CBS 16671）。来自 IST 508 菌株的 D1/D2 结构域和 ITS 序列的系统发育分析表明，它与密切相关。尽管菌株 IST 508 与 CBS 139.77 在 ITS 序列上有 14 个替换和 20 个插入/缺失（6.6%的差异），但在 D1/D2 结构域、线粒体小亚基 rDNA 和细胞色素氧化酶 II 序列上没有发现差异。另一方面，菌株 IST 508 和 CBS 139.77 在 D1/D2 结构域序列上与 NRRL Y-17577 有 8 个替换（1.4%的差异），在细胞色素氧化酶 II 序列上有 16 个替换（2.7%的差异），在线粒体小亚基 rDNA 序列上有 16 个替换（3.7%的差异）。由于和中的可变表型测试数量众多，因此这两个种的菌株难以区分。与其他描述的物种不同，这两个种在微观形态上没有差异。然而，基于这两个菌株（CBS 139.77 和 IST 508）以及 NRRL Y-17577 之间的分子差异及其系统发育分析，CBS 139.77 和 IST 508 菌株属于，并且应该被视为一个独立的种，而不是的同义词。我们提出了的修正描述。
