Cellular Therapy and Hematopoietic Transplant Unit, Hematology Department, Virgen de la Arrixaca Clinical University Hospital, IMIB-Arrixaca, University of Murcia, Murcia, Spain.
Special Care in Dentistry and Gerodontology, Faculty of Medicine, School of Dentistry, University of Murcia, Murcia, Spain.
Int Endod J. 2019 Aug;52(8):1196-1209. doi: 10.1111/iej.13107. Epub 2019 Mar 28.
To analyse in vitro changes in ion release and biological properties of Endocem-MTA (Maruchi, Wonju, Korea) and NeoMTA-Plus (Avalon Biomed Inc, Bradenton, FL, USA) exposed to acidic or neutral environment on human dental periodontal ligament stem cells (hPDLSCs).
Cell viability and wound healing assays were performed using eluates of each material. Cell death and changes in phenotype induced by the set endodontic sealer eluates were evaluated through flow cytometry. To evaluate cell attachment to the different materials, hPDLSCs were directly seeded onto the material surfaces and analysed by scanning electron microscopy. The chemical composition of the materials was determined by energy-dispersive X-ray (EDX), and ion release was evaluated by inductively coupled plasma-mass spectrometry. Statistical analysis was performed with analysis of variance and a Bonferroni or Tukey post-test (α < 0.05).
The MTT assay revealed non-cytotoxic effects of NeoMTA-Plus and Endocem-MTA at pH 5.2 and 7.4. However, there were minor differences compared with the control, especially at pH 5.2, where both materials were associated with significantly greater cell viability (P < 0.05). In both environments, the materials stimulated hPDLSCs to migrate. hPDLSCs were attached to the bioactive cements, with multiple prolongations proliferated on the surface of the samples. Moreover, there were no changes to cell phenotype or apoptosis/necrosis rates, indicating that the acidic environment did not induce cell death. Prismatic crystalline structures were seen on the surface of the cements exposed to butyric acid and EDX analysis identified a marked peak of Ca from NeoMTA-Plus and Endocem-MTA in acidic and physiological environments.
An acidic environment favoured the release of Ca ions from both bioactive cements, and the cytotoxicity of these bioactive cements was low in both environments studied.
分析在酸性或中性环境下，Endocem-MTA（韩国务川 Maruchi）和 NeoMTA-Plus（美国佛罗里达州 Bradenton 的 Avalon Biomed Inc）暴露于人类牙周膜干细胞（hPDLSCs）时离子释放和生物学特性的体外变化。
使用各材料的浸提液进行细胞活力和划痕愈合试验。通过流式细胞术评估设定的根管封闭剂浸提液诱导的细胞死亡和表型变化。为了评估细胞对不同材料的附着，将 hPDLSCs 直接接种到材料表面，并用扫描电子显微镜进行分析。通过能量色散 X 射线（EDX）测定材料的化学成分，并通过电感耦合等离子体质谱法评估离子释放。采用方差分析和 Bonferroni 或 Tukey 后检验（α<0.05）进行统计分析。
在 pH 值为 5.2 和 7.4 时，MTT 测定显示 NeoMTA-Plus 和 Endocem-MTA 无细胞毒性作用。然而，与对照组相比，差异较小，特别是在 pH 值为 5.2 时，两种材料的细胞活力均显著增加（P<0.05）。在两种环境下，材料均刺激 hPDLSCs 迁移。hPDLSCs 附着在生物活性水泥上，在样品表面有多个延长的突起。此外，细胞表型或凋亡/坏死率没有变化，表明酸性环境不会诱导细胞死亡。在暴露于丁酸的水泥表面观察到棱柱形结晶结构，EDX 分析在酸性和生理环境中均鉴定出 NeoMTA-Plus 和 Endocem-MTA 的 Ca 明显峰值。
酸性环境有利于两种生物活性水泥中 Ca 离子的释放，并且在研究的两种环境中，这些生物活性水泥的细胞毒性均较低。
