Silva Emmanuel João Nogueira Leal, Carvalho Nancy Kudsi de, Ronconi Carina Taboada, De-Deus Gustavo, Zuolo Mario Luis, Zaia Alexandre Augusto
Department of Endodontics, Health Sciences Center, UNIGRANRIO - Universidade Grande Rio, Rio de Janeiro, RJ, Brazil.
Department of Endodontics, Piracicaba Dental School, UNICAMP - Universidade Estadual de Campinas, Piracicaba, SP, Brazil.
Braz Dent J. 2016 Oct-Dec;27(6):652-656. doi: 10.1590/0103-6440201600792.
The aim of the present study was to evaluate the cytotoxic effects of five endodontic sealers (AH Plus, Endomethasone N, EndoSequence BC, MTA Fillapex and Pulp Canal Sealer EWT) using a three-dimensional (3D) cell culture model. A conventional bi-dimensional (2D) cell culture model was used as reference technique for comparison. Balb/c 3T3 fibroblasts were cultured in conventional bi-dimensional cell culture and in rat-tail collagen type I three-dimensional cell culture models. Then, both cell cultures were incubated with elutes of freshly mixed endodontic sealers for 24 h. Cell viability was measured by the methyl-thiazol-diphenyltetrazolium assay (MTT). Data were statistically analyzed using ANOVA and the Tukey test at a significance level of p<0.05. All tested sealers exhibited cytotoxic effects; however, cytotoxic effect was culture model- and sealer-dependent. Sealers showed higher cytotoxicity in 2D than in 3D cell culture model (p<0.05). In both conditions, EndoSequence BC showed the lowest cytotoxicity (p<0.05). MTA Fillapex was much more cytotoxic than the other tested endodontic sealers (p<0.05), with the exception of AH Plus in the 2D cell culture model (p>0.05). Endomethasone N and Pulp Canal Sealer EWT showed lower cytotoxic effects than AH Plus in 2D cell culture model (p<0.05); however no statistical differences was observed among these sealers in 3D cell culture model. It may be concluded that cytotoxicity was higher in 2D cell culture compared to 3D cell culture. EndoSequence BC sealer exhibited the highest cytocompatibility and MTA Fillapex the lowest cytocompatibility.
本研究的目的是使用三维（3D）细胞培养模型评估五种根管封闭剂（AH Plus、Endomethasone N、EndoSequence BC、MTA Fillapex和牙髓根管封闭剂EWT）的细胞毒性作用。使用传统的二维（2D）细胞培养模型作为比较的参考技术。将Balb/c 3T3成纤维细胞培养在传统的二维细胞培养和大鼠尾I型胶原三维细胞培养模型中。然后，将两种细胞培养物与新混合的根管封闭剂洗脱液孵育24小时。通过甲基噻唑二苯基四氮唑试验（MTT）测量细胞活力。使用方差分析（ANOVA）和Tukey检验对数据进行统计学分析，显著性水平为p<0.05。所有测试的封闭剂均表现出细胞毒性作用；然而，细胞毒性作用取决于培养模型和封闭剂。封闭剂在二维细胞培养中比在三维细胞培养模型中表现出更高的细胞毒性（p<0.05）。在两种情况下，EndoSequence BC表现出最低的细胞毒性（p<0.05）。MTA Fillapex的细胞毒性比其他测试的根管封闭剂高得多（p<0.05），二维细胞培养模型中的AH Plus除外（p>0.05）。在二维细胞培养模型中，Endomethasone N和牙髓根管封闭剂EWT的细胞毒性作用低于AH Plus（p<0.05）；然而，在三维细胞培养模型中，这些封闭剂之间未观察到统计学差异。可以得出结论，与三维细胞培养相比，二维细胞培养中的细胞毒性更高。EndoSequence BC封闭剂表现出最高的细胞相容性，而MTA Fillapex表现出最低的细胞相容性。
