Department of Biomedical Engineering, University of Victoria, Victoria, BC V8W 2Y2, Canada.
Department of Mechanical Engineering, University of Victoria, Victoria, BC V8W 2Y2, Canada.
STAR Protoc. 2022 Apr 22;3(2):101348. doi: 10.1016/j.xpro.2022.101348. eCollection 2022 Jun 17.
3D bioprinting-a type of additive manufacturing-can create 3D tissue constructs resembling tissues. Here, we present a protocol for 3D printing neural tissues using Axolotl Biosciences' fibrin-based bioink and the CELLINK BIO X bioprinter with a pneumatic printhead. This workflow can be applied to printing 3D tissue models using a variety of cell lines and any chemically crosslinked bioink. These 3D-printed tissue models can be used for applications such as drug screening and disease modeling .
3D 生物打印——一种增材制造技术——可以创建类似于组织的 3D 组织构建体。在这里，我们展示了一种使用 Axolotl Biosciences 的纤维蛋白基生物墨水和 CELLINK BIO X 带有气动打印头的生物打印机进行 3D 打印神经组织的方案。该工作流程可用于使用各种细胞系和任何化学交联生物墨水打印 3D 组织模型。这些 3D 打印的组织模型可用于药物筛选和疾病建模等应用。
