Al-Deen Said Sayf, Jatana Samreen, Ponti András K, Johnson Erin E, Such Kimberly A, Zangara Megan T, Madajka Maria, Papay Francis, McDonald Christine
Department of Inflammation & Immunity, Lerner Research Institute, Cleveland Clinic, Cleveland, OH 44195, USA.
Department of Biology, John Carroll University, University Heights, OH 44118, USA.
J Biol Methods. 2022 Feb 21;9(1):e158. doi: 10.14440/jbm.2022.379. eCollection 2022.
Severe burns are traumatic and physically debilitating injuries with a high rate of mortality. Bacterial infections often complicate burn injuries, which presents unique challenges for wound management and improved patient outcomes. Currently, pigs are used as the gold standard of pre-clinical models to study infected skin wounds due to the similarity between porcine and human skin in terms of structure and immunological response. However, utilizing this large animal model for wound infection studies can be technically challenging and create issues with data reproducibility. We present a detailed protocol for a porcine model of infected burn wounds based on our experience in creating and evaluating full thickness burn wounds infected with on six pigs. Wound healing kinetics and bacterial clearance were measured over a period of 27 d in this model. Enumerated are steps to achieve standardized wound creation, bacterial inoculation, and dressing techniques. Systematic evaluation of wound healing and bacterial colonization of the wound bed is also described. Finally, advice on animal housing considerations, efficient bacterial plating procedures, and overcoming common technical challenges is provided. This protocol aims to provide investigators with a step-by-step guide to execute a technically challenging porcine wound infection model in a reproducible manner. Accordingly, this would allow for the design and evaluation of more effective burn infection therapies leading to better strategies for patient care.
严重烧伤是具有高死亡率的创伤性和身体衰弱性损伤。细菌感染常使烧伤复杂化，这给伤口管理和改善患者预后带来了独特挑战。目前，由于猪和人类皮肤在结构和免疫反应方面具有相似性，猪被用作研究感染性皮肤伤口的临床前模型的金标准。然而，利用这种大型动物模型进行伤口感染研究在技术上具有挑战性，并会产生数据可重复性问题。基于我们创建和评估六只感染了[具体细菌名称未给出]的全层烧伤伤口的经验，我们提出了一种用于感染性烧伤伤口猪模型的详细方案。在此模型中，在27天的时间内测量伤口愈合动力学和细菌清除情况。列举了实现标准化伤口创建、细菌接种和敷料技术的步骤。还描述了对伤口愈合和伤口床细菌定植的系统评估。最后，提供了关于动物饲养考虑、高效细菌接种程序以及克服常见技术挑战的建议。本方案旨在为研究人员提供一份逐步指南，以可重复的方式执行具有技术挑战性的猪伤口感染模型。因此，这将有助于设计和评估更有效的烧伤感染治疗方法，从而为患者护理制定更好的策略。
