α-蒎烯对大鼠胃损伤中 Nrf2-HO1 信号通路的影响。

The effects of alpha-pinene on the Nrf2-HO1 signaling pathway in gastric damage in rats.

机构信息

Department of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

出版信息

Mol Biol Rep. 2023 Oct;50(10):8615-8622. doi: 10.1007/s11033-023-08765-y. Epub 2023 Aug 31.

DOI:10.1007/s11033-023-08765-y

PMID:37648947

Abstract

BACKGROUND

Alpha-pinene (α-pinene) is a monoterpene with gastroprotective activity. We evaluated the gastroprotective effect of α-pinene in the gastric damage model with ethanol.

METHODS

We evaluated the macroscopic evaluation of the stomach cavity, alteration in pH, mRNA expression of nuclear factor erythroid 2- related factor 2 (Nrf2) and heme oxygenase-1 (HO-1), total antioxidant capacity (TAC) levels, and histopathological changes.

RESULTS

Pretreatment with α-pinene (10, 50 and 100 mg/kg i.p.) before oral administration of ethanol reduced gastric mucosal damage by increasing the percentage of ulcer inhibition. Alpha-pinene also increased gastric pH similar to omeprazole. In addition, the histopathological examination showed that in the groups pretreated with α-pinene 50 and 100 mg/kg, and omeprazole20 mg/kg, the lesions were less than the control group. Moreover, α- pinene 10, 50, 100, and omeprazole 20 mg/kg upregulated the NRF2 and HO1.

CONCLUSIONS

Our results show that pretreatment with α-pinene is effective in reducing ethanol-induced gastric damage through regulation of Nrf2/HO-1.

摘要

背景

α-蒎烯（α-pinene）是一种具有胃保护活性的单萜烯。我们评估了α-蒎烯在乙醇致胃损伤模型中的胃保护作用。

方法

我们评估了胃腔的宏观评价、pH 值变化、核因子红细胞 2 相关因子 2（Nrf2）和血红素加氧酶 1（HO-1）的 mRNA 表达、总抗氧化能力（TAC）水平和组织病理学变化。

结果

在口服乙醇前用 α-蒎烯（10、50 和 100mg/kg 腹腔注射）预处理可通过增加溃疡抑制率来减轻胃黏膜损伤。α-蒎烯还可增加胃 pH 值，类似于奥美拉唑。此外，组织病理学检查显示，在 α-蒎烯 50 和 100mg/kg 预处理组和奥美拉唑 20mg/kg 预处理组中，病变小于对照组。此外，α-蒎烯 10、50、100mg/kg 和奥美拉唑 20mg/kg 可上调 NRF2 和 HO1。