Department of Biology, College of Science, Shantou University, Shantou 515063, PR China.
Department of Chemical Engineering, Jieyang Polytechnic, Jieyang, Guangdong 522000, PR China.
Int J Syst Evol Microbiol. 2022 May;72(5). doi: 10.1099/ijsem.0.005357.
A novel Gram-stain-negative, aerobic, non-motile, rod-shaped and yellow-pigmented bacterial strain, designated HU1-3, was isolated from in China. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain HU1-3 represented a member of the genus within the family , phylum , and showed the highest 16S rRNA gene sequence similarity to T58 (97.6 %). Strain HU1-3 grew at 15-35 °C (optimum, 25-30 °C), pH 6.0-8.0 (optimum, pH 7.0) and in the presence of 2-6 % (w/v) NaCl (optimum, 2-4 %). The draft genome of strain HU1-3 comprised 4.1 Mbp with a G+C content of 33.9mol%. Compared with the reference strain CNU004, the average nucleotide identity value of strain HU1-3 was 77.9 %. The major fatty acids (>10 % of the total) were iso-C, iso G-C and iso-C 3-OH. MK-6 was the major respiratory quinone. The major polar lipids were phosphatidylethanolamine, two unidentified aminolipids, and three unknown polar lipids. Many genes that encode glycoside hydrolases (e.g. glucosidase, xylosidase, mannosidase, galactosidase, .) were annotated in the genome of strain HU1-3, which indicated that it might have the ability to degrade various kinds of polysaccharides. Given the phylogenetic, chemotaxonomic, biochemical and genomic data, strain HU1-3 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is HU1-3 (=KCTC 82511=MCCC 1K05798).
一株新型革兰氏阴性、需氧、非运动、杆状和黄色素产生细菌菌株，被命名为 HU1-3，从中国的 中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 HU1-3代表 科 属的一个成员， 门，并且与 T58（97.6%）具有最高的 16S rRNA 基因序列相似性。菌株 HU1-3在 15-35°C（最佳温度为 25-30°C）、pH 6.0-8.0（最佳 pH 为 7.0）和 2-6%（w/v）NaCl（最佳浓度为 2-4%）的条件下生长。菌株 HU1-3的基因组草图由 4.1 Mbp 组成，G+C 含量为 33.9mol%。与参考菌株 CNU004 相比，菌株 HU1-3 的平均核苷酸同一性值为 77.9%。主要脂肪酸（占总脂肪酸的>10%）为 iso-C、iso G-C 和 iso-C 3-OH。MK-6 是主要的呼吸醌。主要的极性脂类是磷脂酰乙醇胺、两种未鉴定的胺脂类和三种未知极性脂类。在菌株 HU1-3 的基因组中注释了许多编码糖苷水解酶（如葡萄糖苷酶、木糖苷酶、甘露糖苷酶、半乳糖苷酶等）的基因，这表明它可能具有降解各种多糖的能力。鉴于系统发育、化学分类学、生化和基因组数据，菌株 HU1-3 被认为代表了 属的一个新种，建议将其命名为 sp. nov.。模式菌株为 HU1-3（=KCTC 82511=MCCC 1K05798）。
