Ayob Farah Wahida, Simarani Khanom, Zainal Abidin Nurhayati, Mohamad Jamaludin
Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Microb Biotechnol. 2017 Jul;10(4):926-932. doi: 10.1111/1751-7915.12603. Epub 2017 Jun 14.
This paper reports on the vinca alkaloid produced by a novel Nigrospora sphaerica isolated from Catharanthus roseus. Through liquid chromatography-mass spectrometry (LCMS), only the crude mycelia extract of this fungus was positive for determination of vinblastine. This vinca alkaloid was then purified by using high-performance liquid chromatography (HPLC) and tested for cytotoxicity activity using MTT assays. The breast cell line cancer (MDA-MB 231) was treated with a purified vinblastine which was intracellulary produced by N. sphaerica. The purified vinblastine from extracted leaf of C. roseus was used as a standard comparison. A positive result with a value of half maximal inhibitory concentration (IC ) of > 32 μg ml was observed compared with standard (IC ) of 350 μg ml only. It showed that a vinblastine produced by N. sphaerica has a high cytotoxicity activity even though the concentration of vinblastine produced by this endophytic fungus was only 0.868 μg ml .
本文报道了从长春花中分离出的一种新型球形黑孢菌产生的长春花生物碱。通过液相色谱 - 质谱联用（LCMS），仅该真菌的粗菌丝体提取物对长春碱的测定呈阳性。然后使用高效液相色谱（HPLC）对该长春花生物碱进行纯化，并使用MTT法测试其细胞毒性活性。用球形黑孢菌细胞内产生的纯化长春碱处理乳腺癌细胞系（MDA - MB 231）。将从长春花提取叶中得到的纯化长春碱用作标准对照。与仅350μg/ml的标准对照相比，观察到阳性结果，其半数最大抑制浓度（IC）值> 32μg/ml。结果表明，球形黑孢菌产生的长春碱具有高细胞毒性活性，尽管这种内生真菌产生的长春碱浓度仅为0.868μg/ml。
