Chen Xin, Wang Jinyue, He Zhixian, Liu Xin, Liu Huawei, Wang Xing
College of Medicine, Southwest Jiaotong University, Chengdu, China.
Department of Laboratory Medicine, The Affiliated Hospital of Southwest Jiaotong University, The Third People's Hospital of Chengdu, Chengdu, China.
Front Microbiol. 2022 Apr 19;13:841662. doi: 10.3389/fmicb.2022.841662. eCollection 2022.
This study investigated the effects of gastrodin (GAS) on analgesic, anxiolytic, ferroptosis, and jejunal microbiota in chronic inflammatory pain mice. The chronic inflammatory pain model of C57BL/6J mice was established by hindpaw injection of complete Freund's adjuvant (CFA). After GAS treatment, thermal hyperalgesia test, mechanical allodynia test, elevated plus-maze (EPMT), and open-field test (OFT) were performed to assess the behavioral changes of pain and anxiety. mRNAs of FTHI, GPX4, HO-1, and PTGS2 and jejunal microbiota were measured by qPCR. In CFA-injected C57BL/6 mice, we found that the mechanical and thermal pain threshold were increased with treatment of GAS. In EPMT, the number of entries in open arms and retention times of open arms were increased by GAS. In the OFT, the time spent in the central area was also increased. Furthermore, GAS enhanced mRNA expressions of FTHI, GPX4, and HO-1 but decreased the expression of PTGS2 in a dose-dependent manner. GAS is effective in the treatment of mice chronic inflammatory pain and anxiety-like behaviors. It may be exhibits potential neuroprotective effects through inhibition of ferroptosis independently of the intestinal microbiota.
本研究探讨了天麻素（GAS）对慢性炎性疼痛小鼠的镇痛、抗焦虑、铁死亡及空肠微生物群的影响。通过后爪注射完全弗氏佐剂（CFA）建立C57BL/6J小鼠慢性炎性疼痛模型。GAS治疗后，进行热痛觉过敏试验、机械性异常性疼痛试验、高架十字迷宫试验（EPMT）和旷场试验（OFT），以评估疼痛和焦虑的行为变化。通过qPCR检测FTHI、GPX4、HO-1和PTGS2的mRNA以及空肠微生物群。在注射CFA的C57BL/6小鼠中，我们发现GAS治疗可提高机械性和热痛阈值。在EPMT中，GAS增加了开放臂的进入次数和开放臂的停留时间。在OFT中，中央区域的停留时间也增加了。此外，GAS以剂量依赖性方式增强了FTHI、GPX4和HO-1的mRNA表达，但降低了PTGS2的表达。GAS对小鼠慢性炎性疼痛和焦虑样行为的治疗有效。它可能通过独立于肠道微生物群抑制铁死亡而发挥潜在的神经保护作用。
