Nakamura Jin, Endo Kanta, Sugawara-Narutaki Ayae, Ohtsuki Chikara
Institute for Advanced Research, Nagoya University Furo-cho, Chikusa-ku Nagoya 464-8603 Japan
Department of Materials Chemistry, Graduate School of Engineering, Nagoya University Furo-cho, Chikusa-ku Nagoya 464-8603 Japan
RSC Adv. 2020 Sep 30;10(59):36051-36057. doi: 10.1039/d0ra04924g. eCollection 2020 Sep 28.
This study aims to evaluate the cytocompatibility of layered zirconium phosphate (ZP) and its derivative material that was organically modified using glycerophosphate (ZGP). The ZP and ZGP particles were prepared a reflux method in an aqueous solution containing phosphoric acid. The field emission scanning electron microscopy showed the prepared samples were fine particles with 70-100 nm diameter. X-ray diffraction and Raman spectrometry indicated the presence of a layered crystal structure. The interlayer distance of ZP was estimated to be 0.76 nm from the 002 diffraction. Modification of ZP with β-glycerophosphate, lead to expansion of the interlayer distance of 0.85 nm. Grazing incidence X-ray diffraction and Raman spectrometry showed that the crystal structures of ZP and ZGP were maintained even after the samples were coated onto polyethylene (PE) substrates hot pressing. The water droplet contact angles on the PE substrates coated with the ZP and ZGP particles (ZP/PE and ZGP/PE) were 2 ∼ 6° lesser than that on the uncoated PE substrate. After human adipose-derived stem cells (hASCs) were cultured on the substrates, 2.5-3.5 times higher numbers of adhered cells were observed on the substrates coated with ZP and ZGP than on the uncoated PE substrates and 1.1-1.6 times higher than on the substrate coated with hydroxyapatite particles (HAp/PE). Increasing cell numbers were observed after culturing for 24 h, indicating that the ZP/PE and ZGP/PE showed low cytotoxicity to the hASCs. Furthermore, the ZP/PE showed the highest area of hASC adhesion among all the samples. These results highlight the possibility that layered zirconium phosphate and its organically modified substances can be applied to biomaterials for tissue repair.
本研究旨在评估层状磷酸锆（ZP）及其用甘油磷酸酯有机改性的衍生物材料（ZGP）的细胞相容性。ZP和ZGP颗粒通过在含磷酸的水溶液中采用回流法制备。场发射扫描电子显微镜显示，制备的样品为直径70 - 100 nm的细颗粒。X射线衍射和拉曼光谱表明存在层状晶体结构。根据002衍射，ZP的层间距估计为0.76 nm。用β - 甘油磷酸酯对ZP进行改性，导致层间距扩大至0.85 nm。掠入射X射线衍射和拉曼光谱表明，即使在通过热压将样品涂覆到聚乙烯（PE）基材上之后，ZP和ZGP的晶体结构仍得以保持。涂有ZP和ZGP颗粒的PE基材（ZP/PE和ZGP/PE）上的水滴接触角比未涂覆的PE基材上的水滴接触角小2至6°。在基材上培养人脂肪干细胞（hASC）后，观察到涂有ZP和ZGP的基材上的贴壁细胞数量比未涂覆的PE基材上的高2.5 - 3.5倍，比涂有羟基磷灰石颗粒的基材（HAp/PE）上的高1.1 - 1.6倍。培养24小时后观察到细胞数量增加，表明ZP/PE和ZGP/PE对hASC显示出低细胞毒性。此外，在所有样品中，ZP/PE显示出hASC粘附面积最大。这些结果突出了层状磷酸锆及其有机改性物质可应用于组织修复生物材料的可能性。
