Statistical optimization of photo-induced biofabrication of silver nanoparticles using the cell extract of : insight on characterization and antioxidant potentiality.

Silver nanoparticles were successfully fabricated through a very simple, rapid, one-step photo-induced green approach. The formation of silver nanoparticles was accomplished using the bioactive compounds in the aqueous extract of fresh biomass, which acted as a reducing and capping agent at the same time. The biosynthesis of -silver nanoparticles (O-AgNPs) was investigated under the influence of different light intensities 57.75, 75.90 and 1276.51 μmol m s (bright sunlight). UV-Vis (UV) and Fourier transform infrared (FT-IR) spectroscopy were applied to approve the synthesis of AgNPs. Further, the synthesis process under the exposure to sunlight was adjusted utilizing one factor at a time, and 0.5 mM AgNO concentration, 5 mL solution, pH 6.7 and 30 min sunlight (1276.51 μmol m s) were applied. Furthermore, the central composite design (CCD) was applied to boost the biosynthesis process of O-AgNPs (manufactured at light intensity 75.90 μmol m s). The maximum production of O-AgNPs was attained with 4 detected variables: initial pH level (6.7), AgNO concentration (0.3 mM), extract concentration (3.50 mL) and incubation time (48 h). Moreover, TEM, in addition to SEM, images exposed that the biosynthesized AgNPs were quasi-spherical in shape with a small monodisperse nature, and the size range was between 6.98-23.48 nm in the case of light-induced synthesis (75.90 μmol m s) and 11.58-22.31 nm with sunlight (1276.51 μmol m s).