Li Yongmei, Li Yuemei, Bai Yandong, Wang Rui, Lin Laixiang, Sun Yina
NHC Key Laboratory of Hormones and Development, Tianjin Key Laboratory of Metabolic Diseases, Chu Hsien-I Memorial Hospital & Tianjin Institute of Endocrinology, Tianjin Medical University No. 6 Huanrui North Road, Ruijing Street, Beichen District Tianjin 300134 China
Xiamen Cardiovascular Hospital, Xiamen University No. 2999 Jinshan Road, Huli District Xiamen Fujian 361015 China.
RSC Adv. 2020 Oct 19;10(63):38416-38423. doi: 10.1039/d0ra07466g. eCollection 2020 Oct 15.
Photodynamic therapy (PDT) as a noninvasive technique is widely used to treat cancer diseases due to its low side effects. PDT based on upconversion nanoparticles (UCNPs) improved tissue penetration and photo-stability. However, traditional photosensitizers and UCNPs were difficult to incorporate, which limited the circulation of the UCNPs in blood and decreased the PDT effect. Herein, we designed NaErF@ZnO UCNPs for potential application in thyroid tumor cell PDT. With ZnO coated on NaErF, the blue (415 nm), green (525 nm/545 nm) and red (661 nm) upconversion luminescence enhanced compared with that of NaErF core nanoparticles. Particularly, the generation of UV upconversion emission by NaErF sensitized ZnO, which catalyzed HO and O to produce ROS reactive oxygen species (ROS) to induce papillary thyroid carcinoma (PTC) cell lines BHP 5-16. With 1000 μg mL of NaErF@ZnO UCNPs, the viability of BHP 5-16 cells decreased to about 41% as measured by CCK8 assay with 980 nm NIR irradiation. Moreover, it was confirmed that NaErF@ZnO UCNPs had low toxicity for BHP 5-16 cells. All these results indicated that NaErF@ZnO upconversion nanoparticles were an excellent platform for PDT treatment.
光动力疗法（PDT）作为一种非侵入性技术，因其副作用小而被广泛用于治疗癌症疾病。基于上转换纳米颗粒（UCNPs）的光动力疗法提高了组织穿透性和光稳定性。然而，传统的光敏剂和UCNPs难以结合，这限制了UCNPs在血液中的循环并降低了光动力疗法的效果。在此，我们设计了NaErF@ZnO UCNPs用于甲状腺肿瘤细胞光动力疗法的潜在应用。在NaErF上包覆ZnO后，与NaErF核纳米颗粒相比，蓝色（415 nm）、绿色（525 nm/545 nm）和红色（661 nm）上转换发光增强。特别地，NaErF敏化的ZnO产生紫外上转换发射，催化HO和O产生活性氧（ROS）以诱导甲状腺乳头状癌（PTC）细胞系BHP 5-16。用1000 μg mL的NaErF@ZnO UCNPs，在980 nm近红外照射下通过CCK8测定法测得BHP 5-16细胞的活力降至约41%。此外，证实NaErF@ZnO UCNPs对BHP 5-16细胞具有低毒性。所有这些结果表明NaErF@ZnO上转换纳米颗粒是光动力疗法治疗的一个优秀平台。
