荚膜红假单胞菌谷氨酰胺营养缺陷型中固氮酶活性的去阻遏作用
Derepression of nitrogenase activity in glutamine auxotrophs of Rhodopseudomonas capsulata.
作者信息
Wall J D, Gest H
出版信息
J Bacteriol. 1979 Mar;137(3):1459-63. doi: 10.1128/jb.137.3.1459-1463.1979.
Abstract
In contrast to wild-type cells, glutamine auxotrophs of the photosynthetic bacterium Rhodopseudomonas capsulata synthesize nitrogenase, produce H2 (catalyzed by nitrogenase), and continue to reduce dinitrogen to ammonia in the presence of exogenous NH4+. The glutamine synthetase activity of such mutants is less than 2% of that observed in the wild type. It appears that glutamine synthetase plays a significant role in regulation of nitrogenase synthesis in R. capsulata.
摘要
与野生型细胞相比，光合细菌荚膜红假单胞菌的谷氨酰胺营养缺陷型合成固氮酶、产生氢气（由固氮酶催化），并且在存在外源NH4+的情况下继续将氮气还原为氨。这类突变体的谷氨酰胺合成酶活性不到野生型的2%。看来谷氨酰胺合成酶在荚膜红假单胞菌固氮酶合成的调控中起重要作用。
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Change in subunit composition of the iron protein of nitrogenase from Rhodospirillum rubrum during activation and inactivation of iron protein.
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Expression of the activating enzyme and Fe protein of nitrogenase from Rhodospirillum rubrum.
J Bacteriol. 1982 Nov;152(2):786-91. doi: 10.1128/jb.152.2.786-791.1982.
6
Effect of L-methionine-DL-sulphoximine on the photoproduction of hydrogen by Rhodospirillum rubrum.
Experientia. 1980 Oct 15;36(10):1166-7. doi: 10.1007/BF01976104.
7
Mapping of Rhodopseudomonas capsulata nif genes.
J Bacteriol. 1984 May;158(2):404-10. doi: 10.1128/jb.158.2.404-410.1984.
8
Overproduction of nitrogenase by nitrogen-limited cultures of Rhodopseudomonas palustris.
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10
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本文引用的文献
1
Photoproduction of Molecular Hydrogen by Rhodospirillum rubrum.
Science. 1949 Jun 3;109(2840):558-9. doi: 10.1126/science.109.2840.558.
2
'Glutamine(amide):2-oxoglutarate amino transferase oxido-reductase (NADP); an enzyme involved in the synthesis of glutamate by some bacteria.
J Gen Microbiol. 1970 Dec;64(2):187-94. doi: 10.1099/00221287-64-2-187.
3
Multiple molecular forms of glutamine synthetase produced by enzyme catalyzed adenylation and deadenylylation reactions.
Adv Enzyme Regul. 1970;8:99-118. doi: 10.1016/0065-2571(70)90011-7.
4
Regulation of glutamine synthetase. X. Effect of growth conditions on the susceptibility of Escherichia coli glutamine synthetase to feedback inhibition.
J Bacteriol. 1967 Oct;94(4):949-57. doi: 10.1128/jb.94.4.949-957.1967.
5
Genetic recombination in Rhodopseudomonas capsulata.
Proc Natl Acad Sci U S A. 1974 Mar;71(3):971-3. doi: 10.1073/pnas.71.3.971.
6
Glutamine synthetase and ammonium regulation of nitrogenase synthesis in Klebsiella.
Nature. 1974 Oct 11;251(5475):481-5. doi: 10.1038/251481a0.
7
Map of genes for carotenoid and bacteriochlorophyll biosynthesis in Rhodopseudomonas capsulata.
J Bacteriol. 1976 May;126(2):619-29. doi: 10.1128/jb.126.2.619-629.1976.
8
Genetic transfer of nitrogenase-hydrogenase activity in Rhodopseudomonas capsulata.
Nature. 1975 Dec 18;258(5536):630-1. doi: 10.1038/258630a0.
9
Gene transfer agents, bacteriophages, and bacteriocins of Rhodopseudomonas capsulata.
Arch Microbiol. 1975 Nov 7;105(3):217-24. doi: 10.1007/BF00447140.
10
Regulation and genetics of bacterial nitrogen fixation.
Annu Rev Microbiol. 1975;29:109-29. doi: 10.1146/annurev.mi.29.100175.000545.
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