Kobatake Reiko, Doi Kazuya, Kubo Takayasu, Makihara Yusuke, Oki Yoshifumi, Yokoi Miyuki, Umehara Hanako, Tsuga Kazuhiro
Department of Advanced Prosthodontics, Hiroshima University Graduate School of Biomedical and Health Sciences 1-2-3, Kasumi Minami-ku, Hiroshima 734-8553 Japan
RSC Adv. 2019 Jan 4;9(3):1625-1631. doi: 10.1039/c8ra08744j. eCollection 2019 Jan 9.
The purpose of this study was to develop a novel porous titanium material with superior mechanical strength and osteoconduction for bone reconstruction. Porous titanium samples were fabricated by titanium-slurry impregnate to prepare urethane forms with several porosities (high-porosity; 92%, middle-porosity; 85% and low-porosity; 65%). Porous HA (mean porosity; 75.3%) was used as a control. To evaluate the characteristics of these materials, we performed porosity measurements, scanning electron microscopy (SEM), three-point bending testing, and cell proliferation assays. To evaluate the osteoconduction ability, porous titanium was placed into the femurs of rabbits and histological and histomorphometric evaluations were performed after 3 weeks. In SEM images, porous three-dimensional structures were observed in all samples. The bending strength significantly increased as porosity increased (Ti-65 > Ti-85 > porous HA > Ti-92, < 0.05; respectively). Ti-65, Ti-85, and porous HA showed good cell proliferation. Newly formed bone was observed in the central portion of Ti-65, Ti-85, and porous HA. Ti-92 was mainly detected in the bone marrow tissue. The bone formation areas of Ti-65, Ti-85, and porous HA were significantly higher than that of Ti-92 ( < 0.05). It was suggested that novel developed porous titanium composed of Ti-65 and Ti-85 showed superior mechanical strength and osteoconduction.
本研究的目的是开发一种具有优异机械强度和骨传导性的新型多孔钛材料用于骨重建。通过钛浆料浸渍制备具有几种孔隙率（高孔隙率；92%，中孔隙率；85%和低孔隙率；65%）的聚氨酯模板来制造多孔钛样品。多孔羟基磷灰石（平均孔隙率；75.3%）用作对照。为了评估这些材料的特性，我们进行了孔隙率测量、扫描电子显微镜（SEM）、三点弯曲测试和细胞增殖测定。为了评估骨传导能力，将多孔钛植入兔股骨中，并在3周后进行组织学和组织形态计量学评估。在SEM图像中，所有样品均观察到多孔三维结构。随着孔隙率增加，弯曲强度显著增加（Ti - 65 > Ti - 85 > 多孔羟基磷灰石 > Ti - 92，P < 0.05；分别）。Ti - 65、Ti - 85和多孔羟基磷灰石显示出良好的细胞增殖。在Ti - 65、Ti - 85和多孔羟基磷灰石的中央部分观察到新形成的骨。Ti - 92主要在骨髓组织中检测到。Ti - 65、Ti - 85和多孔羟基磷灰石的骨形成面积显著高于Ti - 92（P < 0.05）。结果表明，新开发的由Ti - 65和Ti - 85组成的多孔钛具有优异的机械强度和骨传导性。
