Yang J, Chen H J, Zhu X D, Vaidya S, Xiang Z, Fan Y J, Zhang X D
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu, 610064, China.
J Mater Sci Mater Med. 2014 Jul;25(7):1747-56. doi: 10.1007/s10856-014-5202-8. Epub 2014 Mar 26.
Repair of load-bearing bone defects remains a challenge in the field of orthopaedic surgery. In the current study, a surface microstructured porous titanium (STPT) successively treated with H2O2/TaCl5 solution and simulated body fluid was used to repair the critical-sized segmental bone defects in rabbit femur, and non-treated porous titanium (NTPT) and porous biphasic calcium phosphate ceramics (PBCP) were used as control, respectively. A 15 mm long implant was positioned in the femoral defect and stabilized by a plate and screws fixation. After implantation into the body for 1, 3 and 6 months, X-ray observation confirmed that porous titanium groups (NTPT and STPT) provided better mechanical support than PBCP group at the early stage. However, there was no obvious difference in the formed bony callus between PBCP and STPT groups in the later stage, and they both showed better shape of bony callus than NTPT group. Micro-CT and histomorphometric analysis for the samples of 6-month implantation demonstrated that more new bone formed in the inner pores of PBCP and STPT groups than that in NTPT group. Moreover, the biomechanical tests revealed that STPT group could bear larger compressive load than NTPT and PBCP groups, almost reaching the level of the normal rabbit femur. STPT exhibited the enhanced repairing effect on the critical-sized segmental bone defect in rabbit femur, meaning that it could be an ideal material for the repair of large bone defect in load-bearing site.
承重骨缺损的修复在骨科手术领域仍然是一项挑战。在本研究中，用H2O2/TaCl5溶液和模拟体液依次处理的表面微结构多孔钛（STPT）用于修复兔股骨临界尺寸节段性骨缺损，未处理的多孔钛（NTPT）和多孔双相磷酸钙陶瓷（PBCP）分别用作对照。将一个15毫米长的植入物置于股骨缺损处，并用钢板和螺钉固定进行稳定。植入体内1、3和6个月后，X线观察证实，在早期多孔钛组（NTPT和STPT）比PBCP组提供了更好的机械支撑。然而，在后期PBCP组和STPT组之间形成的骨痂没有明显差异，并且它们都比NTPT组显示出更好的骨痂形态。对植入6个月的样本进行的Micro-CT和组织形态计量学分析表明，PBCP组和STPT组内部孔隙中形成的新骨比NTPT组更多。此外，生物力学测试显示，STPT组比NTPT组和PBCP组能承受更大的压缩载荷，几乎达到正常兔股骨的水平。STPT对兔股骨临界尺寸节段性骨缺损表现出增强的修复效果，这意味着它可能是修复承重部位大骨缺损的理想材料。
