Lin Sifan, Ke Ze, Liu Kunxing, Zhu Siqi, Li Zhen, Yin Hao, Chen Zhenqiang
Guangdong Provincial Key Laboratory of Optical Fiber Sensing and Communications, Guangzhou, 510632, China.
Guangdong Provincial Engineering Research Center of Crystal and Laser Technology, Guangzhou, 510632, China.
Biomed Opt Express. 2022 Mar 14;13(4):2082-2090. doi: 10.1364/BOE.451006. eCollection 2022 Apr 1.
Gross chromatin imbalance and high DNA content are distinct features of various types of cancer cells. However, severe inflammation can also produce similar symptoms in cells. In this study, normal, inflammatory, and carcinoma hepatic cells were stained with 4',6-diamidino-2-phenylindole (DAPI) and investigated by hyperspectral microscopy. DAPI is a DNA-sensitive fluorochrome. Therefore, the differences in the cellular DNA of the samples can be revealed by the corresponding fluorescence. Our experimental results demonstrate that although chromosomal disorder and high DNA content both occur in severely inflammatory and carcinoma hepatic cells, there is still a slight difference in their DNA, making their fluorescent intensity and even their spectral shapes distinguishable. Based on these spectral features, we developed a method for the precise identification of normal, inflammatory, and carcinoma hepatic cells in the field of view. The identification accuracy for these three types of cells was 99.8%. We believe that examination that combines DAPI staining with hyperspectral microscopy is a potential method for the identification and investigation of various types of cancer tissues.
粗大染色质失衡和高DNA含量是各类癌细胞的显著特征。然而，严重炎症也可在细胞中产生类似症状。在本研究中，对正常、炎性和癌性肝细胞用4′,6-二脒基-2-苯基吲哚（DAPI）进行染色，并通过高光谱显微镜进行研究。DAPI是一种对DNA敏感的荧光染料。因此，样品中细胞DNA的差异可通过相应的荧光显示出来。我们的实验结果表明，虽然染色体紊乱和高DNA含量在严重炎性和癌性肝细胞中均会出现，但它们的DNA仍存在细微差异，使得它们的荧光强度甚至光谱形状都可区分。基于这些光谱特征，我们开发了一种在视野中精确识别正常、炎性和癌性肝细胞的方法。这三种类型细胞的识别准确率为99.8%。我们认为，将DAPI染色与高光谱显微镜相结合的检测方法是识别和研究各类癌组织的一种潜在方法。
