高光谱显微镜结合DAPI染色用于鉴定肝癌细胞。
Hyperspectral microscopy combined with DAPI staining for the identification of hepatic carcinoma cells.
作者信息
Liu Kunxing, Lin Sifan, Zhu Siqi, Chen Yao, Yin Hao, Li Zhen, Chen Zhenqiang
机构信息
Guangdong Provincial Engineering Research Center of Crystal and Laser Technology, Guangzhou 510632, China.
Department of Optoelectronic Engineering, Jinan University, Guangzhou 510632, China.
出版信息
Biomed Opt Express. 2020 Dec 8;12(1):173-180. doi: 10.1364/BOE.412158. eCollection 2021 Jan 1.
In this study, the DAPI staining is firstly reported for use in the identification of hepatic carcinoma cells based on hyperspectral microscopy. Nuclei in cancer cells usually contain more aneuploidies than that in normal cells, leading to the change of DNA content. Here, we stain hepatic carcinoma tissues and normal hepatic tissues with 4',6-diamidino-2-phenylindole (DAPI) which is sensitive to the DNA content as a fluorochrome binds to DNA. Consequently, the difference in DNA content between hepatic carcinoma cells and normal hepatic cells can be identified by the fluorescent spectral characteristics. Harnessing the hyperspectral microscopy, we find that the fluorescent properties of these two kinds of cells are different not only in the intensity but also in the spectral shape. These properties are exploited to train a support vector machine (SVM) model for classifying cells. The results show that the sensitivity and specificity for the identification of 1000 hepatic carcinoma samples are 99.3% and 99.1%, respectively.
在本研究中，首次报道了基于高光谱显微镜利用4',6-二脒基-2-苯基吲哚（DAPI）染色来鉴定肝癌细胞。癌细胞中的细胞核通常比正常细胞含有更多的非整倍体，导致DNA含量发生变化。在此，我们用对DNA含量敏感的4',6-二脒基-2-苯基吲哚（DAPI）作为荧光染料与DNA结合，对肝癌组织和正常肝组织进行染色。因此，可通过荧光光谱特征来识别肝癌细胞与正常肝细胞之间DNA含量的差异。利用高光谱显微镜，我们发现这两种细胞的荧光特性不仅在强度上不同，在光谱形状上也不同。利用这些特性训练支持向量机（SVM）模型对细胞进行分类。结果表明，对1000个肝癌样本进行鉴定的灵敏度和特异性分别为99.3%和99.1%。
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