Si Xue-Jing, Wang Hong-Ling, Wu Tun-Hua, Wang Ping
School of Pharmaceutical Sciences, Wenzhou Medical University Wenzhou 325035 China
School of Information Engineering, Wenzhou Business College Wenzhou 325035 China.
RSC Adv. 2020 Dec 8;10(71):43256-43261. doi: 10.1039/d0ra05298a. eCollection 2020 Nov 27.
The Maillard reaction and its fluorescent products have attracted widespread attention in the field of food safety and biology. Herein, the novel Maillard reaction fluorescent nanoparticles (MRFNs) as a fluorescent probe were synthesized a "green" method with simple technical processes. In addition, the effects of tetracycline (TC) and chlorotetracycline (CTC) representing certain properties of tetracyclines (TCs) on the fluorescence behaviour of MRFNs were studied, respectively. The present study showed that the fluorescence intensity of MRFNs greatly enhanced with a linear increase in the CTC concentration. However, with the gradual increase in the TC concentration, the intensity of MRFNs tended to significantly decrease linearly. Based on this, novel fluorescence analysis methods for the simple and rapid detection of TC and CTC in water bodies were established, respectively. Significantly, the proposed detection methods were successfully adopted for detecting TC and CTC in some environmental water samples. Besides, the possible mechanisms for TC-induced fluorescence quenching and CTC-induced fluorescence enhancement of MRFNs were also discussed, respectively.
美拉德反应及其荧光产物在食品安全和生物学领域引起了广泛关注。在此，采用技术流程简单的“绿色”方法合成了新型美拉德反应荧光纳米颗粒（MRFNs）作为荧光探针。此外，分别研究了代表四环素类（TCs）某些特性的四环素（TC）和金霉素（CTC）对MRFNs荧光行为的影响。本研究表明，随着CTC浓度呈线性增加，MRFNs的荧光强度显著增强。然而，随着TC浓度的逐渐增加，MRFNs的强度呈线性显著下降趋势。基于此，分别建立了简单快速检测水体中TC和CTC的新型荧光分析方法。值得注意的是，所提出的检测方法已成功用于检测一些环境水样中的TC和CTC。此外，还分别讨论了TC诱导MRFNs荧光猝灭和CTC诱导MRFNs荧光增强的可能机制。
