Skinner M A, Thompson K, Ezaki T, Marbrook J
Br J Cancer. 1987 Mar;55(3):259-63. doi: 10.1038/bjc.1987.50.
A novel tumour system has been used to study the effect of natural killer cells on tumour growth by using agents which modify natural killer cell activity. The tumour cells are hybridoma cells which secrete antibody specific for red blood cells so that tumour growth can be quantitated by a haemolytic plaque assay. Spleen-seeking variants have been derived from original hybrids which are sensitive to natural killer cells. Treatment of mice with polyinosinic-polycytidylic acid substantially enhanced natural killer cell activity and correlated closely with a reduction in the growth of the hybridoma tumour cells in the spleen and life extension. Conversely, a single injection of anti-asialo GM, antibody resulted in a substantial increase in the number of plaque forming splenic tumour cells and virtual elimination of natural killer cell activity. These data demonstrate the important role of natural killer cells in constraining the growth of a tumour of B cell origin and establishes the usefulness of this tumour model in studying the biology of effects on tumour growth.
一种新型肿瘤系统已被用于研究自然杀伤细胞对肿瘤生长的影响，方法是使用改变自然杀伤细胞活性的试剂。肿瘤细胞是杂交瘤细胞，可分泌针对红细胞的特异性抗体，因此肿瘤生长可以通过溶血空斑试验进行定量。已从对自然杀伤细胞敏感的原始杂交瘤中获得了趋脾变体。用聚肌苷酸-聚胞苷酸处理小鼠可显著增强自然杀伤细胞活性，这与脾脏中杂交瘤肿瘤细胞生长的减少和生存期的延长密切相关。相反，单次注射抗唾液酸GM1抗体导致形成空斑的脾肿瘤细胞数量大幅增加，自然杀伤细胞活性几乎完全消除。这些数据证明了自然杀伤细胞在抑制B细胞起源肿瘤生长中的重要作用，并确立了该肿瘤模型在研究肿瘤生长影响生物学方面的实用性。
