Wei W Z, Heppner G
Br J Cancer. 1987 Jun;55(6):589-94. doi: 10.1038/bjc.1987.120.
Tissue infiltrating lymphocytes were isolated from BALB/c line C4 preneoplastic hyperplastic alveolar nodules (HAN) and spontaneous tumours that arose from the HANs. NK activity of the lymphocytic infiltrates was tested in a 4 h chromium release assay using 51Cr labelled YAC cells. In situ lymphocytes of C4 HAN expressed 3-4 fold greater relative lytic activity (Pross et al., 1981) than did normal spleen cells whereas the relative lytic activity of C4 tumour infiltrates was equivalent or less than that of normal spleen cells. Spleen cells of all lesion bearers had reduced cytolytic capacity. YAC cell lysis by spleen cells and HAN infiltrates correlated with increasing E/T ratios. The degree of YAC lysis by C4 tumour infiltrates, however, either decreased, stayed the same, or increased non-exponentially with increasing E/T ratios especially at E/T greater than 50. Indeed, C4 tumour infiltrates from animals pretreated with anti-asialo GM1 (ASGM) could suppress the NK activity of normal spleen cells. The lytic activity of both C4 HAN and tumour infiltrates could be enhanced or depressed by in vivo treatment with poly IC or anti-ASGM, respectively. These results indicate that NK cells are activated or recruited into C4 preneoplastic lesions but their lytic activity wanes and suppressive activity arises with progression to neoplasia.
从BALB/c系C4癌前增生性肺泡结节（HAN）及由HAN发展而来的自发性肿瘤中分离出组织浸润淋巴细胞。使用51Cr标记的YAC细胞，通过4小时铬释放试验检测淋巴细胞浸润物的自然杀伤（NK）活性。C4 HAN的原位淋巴细胞表达的相对溶解活性（Pross等人，1981年）比正常脾细胞高3 - 4倍，而C4肿瘤浸润物的相对溶解活性等于或低于正常脾细胞。所有病变携带者的脾细胞的细胞溶解能力均降低。脾细胞和HAN浸润物对YAC细胞的裂解与E/T比值的增加相关。然而，随着E/T比值增加，尤其是E/T大于50时，C4肿瘤浸润物对YAC细胞的裂解程度要么降低、要么保持不变，要么呈非指数性增加。实际上，用抗唾液酸GM1（ASGM）预处理动物后获得的C4肿瘤浸润物能够抑制正常脾细胞的NK活性。分别用聚肌胞苷酸（poly IC）或抗ASGM进行体内处理，可增强或抑制C4 HAN和肿瘤浸润物的溶解活性。这些结果表明，NK细胞被激活或募集到C4癌前病变中，但随着肿瘤的发展，它们的溶解活性减弱，抑制活性出现。
