Xie Xianzhou, Mao Congyang, Liu Xiangmei, Tan Lei, Cui Zhenduo, Yang Xianjin, Zhu Shengli, Li Zhaoyang, Yuan Xubo, Zheng Yufeng, Yeung Kelvin Wai Kwok, Chu Paul K, Wu Shuilin
School of Materials Science & Engineering, Tianjin University, Tianjin 300072, China.
Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Green Preparation and Application of Functional Materials, Hubei Key Laboratory of Polymer Materials, School of Materials Science & Engineering, Hubei University, Wuhan 430062, China.
ACS Cent Sci. 2018 Jun 27;4(6):724-738. doi: 10.1021/acscentsci.8b00177. Epub 2018 Jun 5.
Bacterial infection and associated complications are threats to human health especially when biofilms form on biomedical devices and artificial implants. Herein, a hybrid polydopamine (PDA)/AgPO/graphene oxide (GO) coating is designed and constructed to achieve rapid bacteria killing and eliminate biofilms in situ. By varying the amount of GO in the hybrid coating, the bandgap can be tuned from 2.52 to 2.0 eV so that irradiation with 660 nm visible light produces bacteria-killing effects synergistically in concert with reactive oxygen species (ROS). GO regulates the release rate of Ag to minimize the cytotoxicity while maintaining high antimicrobial activity, and a smaller particle size enhances the yield of ROS. After irradiation with 660 nm visible light for 15 min, the antimicrobial rates of the PDA/AgPO/GO hybrid coating against and are 99.53% and 99.66%, respectively. In addition, this hybrid coating can maintain a repeatable and sustained antibacterial efficacy. The released Ag and photocatalytic AgPO produce synergistic antimicrobial effects in which the ROS increases the permeability of the bacterial membranes to increase the probability of Ag to enter the cells to kill them together with ROS synergistically.
细菌感染及相关并发症对人类健康构成威胁，尤其是当生物膜在生物医学设备和人工植入物上形成时。在此，设计并构建了一种聚多巴胺（PDA）/磷酸银（AgPO）/氧化石墨烯（GO）复合涂层，以实现快速杀菌并原位消除生物膜。通过改变复合涂层中GO的含量，其带隙可从2.52 eV调节至2.0 eV，从而使660 nm可见光照射与活性氧（ROS）协同产生杀菌效果。GO调节Ag的释放速率，在保持高抗菌活性的同时将细胞毒性降至最低，并且较小的粒径提高了ROS的产量。在660 nm可见光照射15分钟后，PDA/AgPO/GO复合涂层对金黄色葡萄球菌和大肠杆菌的抗菌率分别为99.53%和99.66%。此外，这种复合涂层可保持可重复且持续的抗菌效果。释放出的Ag与光催化的AgPO产生协同抗菌作用，其中ROS增加细菌细胞膜的通透性，提高Ag进入细胞的概率，从而与ROS协同杀死细菌。
