de la Garza Ana Laura, Etxeberria Usune, Haslberger Alexander, Aumueller Eva, Martínez J Alfredo, Milagro Fermín I
1 Department of Nutrition, Food Science and Physiology, University of Navarra, Pamplona , Spain .
2 Centre for Nutrition Research, Faculty of Pharmacy, University of Navarra , Pamplona, Spain .
J Med Food. 2015 Aug;18(8):890-8. doi: 10.1089/jmf.2014.0088. Epub 2015 Jan 19.
Obesity is characterized by an increased production of inflammatory markers. High levels of circulating free fatty acids and chronic inflammation lead to increased oxidative stress, contributing to the development of insulin resistance (IR). Recent studies have focused on the potential use of flavonoids for obesity management due to their antioxidant and anti-inflammatory properties. This study was designed to investigate the antioxidant and anti-inflammatory effects of helichrysum and grapefruit extracts in overweight insulin-resistant rats. Thirty-eight male Wistar rats were randomly distributed in two groups: control group (n=8) and high-fat sucrose (HFS) group (n=30). After 22 days of ad libitum water and food access, the rats fed HFS diet changed to standard diet and were reassigned into three groups (n=10 each group): nonsupplemented, helichrysum extract (2 g/kg bw), and grapefruit extract (1 g/kg bw) administered for 5 weeks. Rats supplemented with both extracts gained less body weight during the 5-week period of treatment, showed lower serum insulin levels and liver TBARS levels. Leptin/adiponectin ratio, as an indicator of IR, was lower in both extract-administered groups. These results were accompanied by a reduction in TNFα gene expression in epididymal adipose tissue and intestinal mucosa, and TLR2 expression in intestinal mucosa. Helichrysum and grapefruit extracts might be used as complement hypocaloric diets in weight loss treatment. Both extracts helped to reduce weight gain, hyperinsulinemia, and IR, improved inflammation markers, and decreased the HFS diet-induced oxidative stress in insulin-resistant rats.
肥胖的特征是炎症标志物产生增加。高水平的循环游离脂肪酸和慢性炎症导致氧化应激增加，促进胰岛素抵抗（IR）的发展。由于黄酮类化合物具有抗氧化和抗炎特性，最近的研究集中在其用于肥胖管理的潜在用途上。本研究旨在调查蜡菊提取物和葡萄柚提取物对超重胰岛素抵抗大鼠的抗氧化和抗炎作用。38只雄性Wistar大鼠随机分为两组：对照组（n = 8）和高脂蔗糖（HFS）组（n = 30）。在随意饮水和进食22天后，喂食HFS饮食的大鼠改为标准饮食，并重新分为三组（每组n = 10）：未补充组、蜡菊提取物组（2 g/kg体重）和葡萄柚提取物组（1 g/kg体重），给药5周。在5周的治疗期间，补充两种提取物的大鼠体重增加较少，血清胰岛素水平和肝脏丙二醛水平较低。作为IR指标的瘦素/脂联素比值在两个提取物给药组中均较低。这些结果伴随着附睾脂肪组织和肠黏膜中TNFα基因表达的降低以及肠黏膜中TLR2表达的降低。蜡菊提取物和葡萄柚提取物可作为补充性低热量饮食用于减肥治疗。两种提取物都有助于减轻体重增加、高胰岛素血症和IR，改善炎症标志物，并降低HFS饮食诱导的胰岛素抵抗大鼠的氧化应激。
