Key Laboratory of Dairy Biotechnology and Engineering (IMAU), Ministry of Education, Inner Mongolia Agricultural University, Hohhot, 010018, PR China.
Key Laboratory of Dairy Products Processing, Ministry of Agriculture and Rural Affairs, Inner Mongolia Agricultural University, Hohhot, 010018, PR China.
Int J Syst Evol Microbiol. 2022 May;72(5). doi: 10.1099/ijsem.0.005359.
Two lactic acid bacterial strains (IMAU80584 and IMAU92037) were isolated from naturally fermented dairy products (kurut and yoghurt) in China and Russia. Based on sequence analysis of the 16S rRNA gene it was revealed that these strains belonged to . However, phylogenetic tree analyses of two housekeeping genes, (encoding RNA polymerase alpha subunit) and (encoding phenylalanyl-tRNA synthase alpha subunit), and 88 core genes, indicated the two strains were separated into an independent monophyletic branch from DSM 19907, forming an infra-specific subgroup. The average nucleotide identity and digital DNA-DNA hybridization values between IMAU80584 and DSM 19907 were 93.1 and 52.8 %, respectively. Strains IMAU80584 and IMAU92037 are distinguished from DSM 19907 because they have different polar lipids and fatty acids. The novel subgroup strains could not ferment gluconate potassium. The DNA G+C content of strain IMAU80584 was 42.3 mol%. The major cellular fatty acids were C, C 9t and summed feature 5 (C ante and/or C 6 and/or C 9c). Therefore, based on the results of polyphasic taxonomic analysis, IMAU80584 and IMAU92037 could be considered as a novel subspecies in the species with the proposed name subsp. subsp. nov. The type strain is IMAU80584 (=GDMCC 1.2566=JCM 34647).
两株乳酸菌（IMAU80584 和 IMAU92037）分别从中国和俄罗斯的自然发酵乳制品（库尔图和酸奶）中分离出来。基于 16S rRNA 基因序列分析，这些菌株属于 。然而，两个看家基因（编码 RNA 聚合酶α亚基）和 （编码苯丙氨酸-tRNA 合成酶α亚基）以及 88 个核心基因的系统发育树分析表明，这两个菌株从 DSM 19907 中分离出来，形成一个独立的单系分支，形成一个亚种内的亚群。IMAU80584 与 DSM 19907 之间的平均核苷酸同一性和数字 DNA-DNA 杂交值分别为 93.1%和 52.8%。菌株 IMAU80584 和 IMAU92037 与 DSM 19907 不同，因为它们具有不同的极性脂质和脂肪酸。新型亚群菌株不能发酵葡萄糖酸钾。菌株 IMAU80584 的 DNA G+C 含量为 42.3mol%。主要的细胞脂肪酸为 C、C 9t 和特征脂肪酸 5（C ante 和/或 C 6 和/或 C 9c）。因此，基于多相分类分析的结果，IMAU80584 和 IMAU92037 可被认为是物种 中的一个新亚种，其名称为 subsp. subsp. nov.。模式菌株为 IMAU80584（=GDMCC 1.2566=JCM 34647）。
