Laboratory of Medical Investigation, LIM-18, Endocrinology Division, School of Medicine, University of São Paulo, São Paulo, Brazil.
Rheumatology Division, School of Medicine, University of São Paulo, São Paulo, Brazil.
Exp Gerontol. 2022 Jul;164:111831. doi: 10.1016/j.exger.2022.111831. Epub 2022 May 4.
This study aimed to evaluate the impact of a 12-week calorie-restricted diet and recreational sports training on gene expressions IL-15, ATROGIN-1 and MURF-1 in skeletal muscle of T2D patients.
Older adults with T2D (n = 39, 60 ± 6.0 years, BMI 33.5 ± 0.6 kg/m) were randomly allocated to Diet+Soccer (DS), Diet+Running (DR) or Diet (D). The training sessions were moderate-to-high-intensity and performed 3 × 40 min/week for 12-weeks. Gene expression from vastus lateralis muscle obtained by qRT-PCR, dual-energy X-ray and fasting blood testing measurements were performed before and after 12-weeks. Statistical analysis adopted were two-way ANOVA and Paired t-test for gene expression, and RM-ANOVA test for the remainder variables.
Total body weight was reduced in ~4 kg representing body fat mass in all groups after 12-weeks (P < 0.05). HbA1c values decreased in all groups post-intervention. Lipids profile improved in the training groups (P < 0.05) after 12-weeks. ATROGIN-1 and MURF-1 mRNA reduced in the DS (1.084 ± 0.14 vs. 0.754 ± 1.14 and 1.175 ± 0.34 vs. 0.693 ± 0.12, respectively; P < 0.05), while IL-15 mRNA increased in the DR (1.056 ± 0.12 vs. 1.308 ± 0.13; P < 0.05) after 12-weeks intervention.
Recreational training with a moderate calorie-restricted diet can downregulates the expression of atrophy-associated myokines and increases the expression of anti-inflammatory gene IL-15.
本研究旨在评估为期 12 周的热量限制饮食和娱乐性运动训练对 2 型糖尿病患者骨骼肌中 IL-15、ATROGIN-1 和 MURF-1 基因表达的影响。
将 39 名年龄较大的 2 型糖尿病患者（60 ± 6.0 岁，BMI 33.5 ± 0.6 kg/m）随机分配到饮食+足球（DS）、饮食+跑步（DR）或饮食（D）组。训练课程为中高强度，每周进行 3 次，每次 40 分钟，共 12 周。在 12 周前后，通过 qRT-PCR、双能 X 射线和空腹血液检测测量获取股外侧肌的基因表达。采用双因素方差分析和配对 t 检验进行基因表达分析，采用重复测量方差分析检验其余变量。
所有组在 12 周后体重减轻约 4kg，代表体脂质量均降低（P<0.05）。所有组的糖化血红蛋白值在干预后均降低。训练组的血脂谱在 12 周后得到改善（P<0.05）。DS 组的 ATROGIN-1 和 MURF-1mRNA 减少（分别为 1.084 ± 0.14 对 0.754 ± 1.14 和 1.175 ± 0.34 对 0.693 ± 0.12；P<0.05），而 DR 组的 IL-15mRNA 增加（1.056 ± 0.12 对 1.308 ± 0.13；P<0.05）。
中等热量限制饮食和娱乐性运动训练可下调与萎缩相关的肌因子表达，并增加抗炎基因 IL-15 的表达。
