Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Shenzhen University, Shenzhen, Guangdong, 518060, PR China.
Guangdong Key Laboratory for Biomedical Measurements and Ultrasound Imaging, School of Biomedical Engineering, Shenzhen University, Shenzhen, Guangdong, 518060, PR China.
Anal Chim Acta. 2022 May 22;1208:339843. doi: 10.1016/j.aca.2022.339843. Epub 2022 Apr 20.
The chemistry of the metal-organic frameworks (MOFs) coating may affect the biological functionality of the encapsulated biomacromolecules in harsh environment. Enzymes encapsulated in hydrophilic MAF-7 can retain high activity in harsh environment. We conducted this study to prepare a non-invasive wearable uircase@MAF-7-based electrochemical sensor that can achieve accurate and sensitive detection of UA levels in sweat by integrating a flexible microfluidic chip and wireless electronic readout device. The flexible microfluidic chip enabled an easy and effective collection of sweat samples. MAF-7 protected enzyme activity by encapsulating uricase. The uricase@MAF-7-based electrochemical sensor enabled the highly sensitive detection of UA in the concentration range of 2 μM-70 μM with a detection limit of as low as 0.34 μM. Additionally, we evaluated the utility of the sensor for monitoring UA levels in real sweat samples by means of a high purine dietary challenge. This personalized wearable sweat sensing device has a potential to be used for monitoring disease-related metabolites in daily life.
金属-有机骨架（MOFs）涂层的化学性质可能会影响在恶劣环境中被包裹的生物大分子的生物功能。亲水性 MAF-7 包裹的酶在恶劣环境中仍能保持高活性。我们进行了这项研究，制备了一种非侵入性、可穿戴的基于 uircase@MAF-7 的电化学传感器，该传感器通过集成柔性微流控芯片和无线电子读出设备，实现了对汗液中 UA 水平的准确、灵敏检测。柔性微流控芯片便于轻松有效地收集汗液样本。MAF-7 通过包裹尿酸酶来保护酶的活性。基于尿酸酶@MAF-7 的电化学传感器能够在 2 μM-70 μM 的浓度范围内对 UA 进行高灵敏度检测，检测限低至 0.34 μM。此外，我们通过高嘌呤饮食挑战评估了该传感器在实际汗液样本中监测 UA 水平的实用性。这种个性化的可穿戴汗液传感装置有望用于日常生活中监测与疾病相关的代谢物。
